CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004701
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vesicular-fusion protein SEC18 
Protein Synonyms/Alias
  
Gene Name
 SEC18 
Gene Synonyms/Alias
 YBR080C; YBR0736 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
106AKAFDLFKYSGKQSYacetylation[1]
128ISFRARGKAVSTVFDacetylation[1]
200ETKGILTKQTQINFFubiquitination[2]
714LDDAGRVKVINELSRubiquitination[2]
732NFNVGIKKTLTNIETubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. 
Sequence Annotation
 NP_BIND 281 288 ATP (Potential).
 NP_BIND 564 571 ATP (Potential).
 MOD_RES 226 226 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Cytoplasm; ER-Golgi transport; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 758 AA 
Protein Sequence
MFKIPGFGKA AANHTPPDMT NMDTRTRHLK VSNCPNNSYA LANVAAVSPN DFPNNIYIII 60
DNLFVFTTRH SNDIPPGTIG FNGNQRTWGG WSLNQDVQAK AFDLFKYSGK QSYLGSIDID 120
ISFRARGKAV STVFDQDELA KQFVRCYESQ IFSPTQYLIM EFQGHFFDLK IRNVQAIDLG 180
DIEPTSAVAT GIETKGILTK QTQINFFKGR DGLVNLKSSN SLRPRSNAVI RPDFKFEDLG 240
VGGLDKEFTK IFRRAFASRI FPPSVIEKLG ISHVKGLLLY GPPGTGKTLI ARKIGTMLNA 300
KEPKIVNGPE ILSKYVGSSE ENIRNLFKDA EAEYRAKGEE SSLHIIIFDE LDSVFKQRGS 360
RGDGTGVGDN VVNQLLAKMD GVDQLNNILV IGMTNRKDLI DSALLRPGRF EVQVEIHLPD 420
EKGRLQIFDI QTKKMRENNM MSDDVNLAEL AALTKNFSGA EIEGLVKSAS SFAINKTVNI 480
GKGATKLNTK DIAKLKVTRE DFLNALNDVT PAFGISEEDL KTCVEGGMML YSERVNSILK 540
NGARYVRQVR ESDKSRLVSL LIHGPAGSGK TALAAEIALK SGFPFIRLIS PNELSGMSES 600
AKIAYIDNTF RDAYKSPLNI LVIDSLETLV DWVPIGPRFS NNILQMLKVA LKRKPPQDRR 660
LLIMTTTSAY SVLQQMDILS CFDNEIAVPN MTNLDELNNV MIESNFLDDA GRVKVINELS 720
RSCPNFNVGI KKTLTNIETA RHDEDPVNEL VELMTQSA 758 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0000045; P:autophagic vacuole assembly; IMP:SGD.
 GO:0043001; P:Golgi to plasma membrane protein transport; IMP:SGD.
 GO:0048211; P:Golgi vesicle docking; IDA:SGD.
 GO:0048219; P:inter-Golgi cisterna vesicle-mediated transport; IMP:SGD.
 GO:0035494; P:SNARE complex disassembly; IDA:SGD.
 GO:0042144; P:vacuole fusion, non-autophagic; IDA:SGD.
 GO:0048280; P:vesicle fusion with Golgi apparatus; IDA:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR009010; Asp_de-COase-like_dom.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR004201; Cdc48_dom2.
 IPR003338; CDC4_N-term_subdom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00004; AAA
 PF02933; CDC48_2
 PF02359; CDC48_N 
SMART
 SM00382; AAA
 SM01072; CDC48_2
 SM01073; CDC48_N 
PROSITE
 PS00674; AAA 
PRINTS