CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006776
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal RNA large subunit methyltransferase J 
Protein Synonyms/Alias
 23S rRNA (adenine(2030)-N6)-methyltransferase; 23S rRNA m6A2030 methyltransferase 
Gene Name
 rlmJ 
Gene Synonyms/Alias
 yhiR; b3499; JW3466 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
30SLIIESLKEKDKPFLacetylation[1]
32IIESLKEKDKPFLYLacetylation[1]
34ESLKEKDKPFLYLDTacetylation[1]
133LLRSEFQKDSRARVEacetylation[1]
141DSRARVEKADGFQQLacetylation[1]
170IDPPYEMKTDYQAVVacetylation[1]
185SGIAEGYKRFATGIYacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Specifically methylates the adenine in position 2030 of 23S rRNA. Nascent 23S rRNA seems to be the natural substrate. Appears to be not necessary for ribosome assembly. Seems to be required for the utilization of extracellular DNA as the sole source of carbon and energy. 
Sequence Annotation
  
Keyword
 Complete proteome; Methyltransferase; Reference proteome; RNA-binding; rRNA processing; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 280 AA 
Protein Sequence
MLSYRHSFHA GNHADVLKHT VQSLIIESLK EKDKPFLYLD THAGAGRYQL GSEHAERTGE 60
YLEGIARIWQ QDDLPAELEA YINVVKHFNR SGQLRYYPGS PLIARLLLRE QDSLQLTELH 120
PSDYPLLRSE FQKDSRARVE KADGFQQLKA KLPPVSRRGL ILIDPPYEMK TDYQAVVSGI 180
AEGYKRFATG IYALWYPVVL RQQIKRMIHD LEATGIRKIL QIELAVLPDS DRRGMTASGM 240
IVINPPWKLE QQMNNVLPWL HSKLVPAGTG HATVSWIVPE 280 
Gene Ontology
 GO:0036307; F:23S rRNA (adenine(2030)-N(6))-methyltransferase activity; IDA:EcoCyc.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0015976; P:carbon utilization; IMP:EcoCyc.
 GO:0070475; P:rRNA base methylation; IDA:UniProtKB. 
Interpro
 IPR002052; DNA_methylase_N6_adenine_CS.
 IPR007473; DUF519. 
Pfam
 PF04378; DUF519 
SMART
  
PROSITE
 PS00092; N6_MTASE 
PRINTS