CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013456
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histone-lysine N-methyltransferase SETMAR 
Protein Synonyms/Alias
 SET domain and mariner transposase fusion gene-containing protein; HsMar1; Metnase; Histone-lysine N-methyltransferase; Mariner transposase Hsmar1 
Gene Name
 SETMAR 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
330RLTLETMKMMLDKKQacetylation[1]
335TMKMMLDKKQIRAIFacetylation[1]
378RTVQWWFKKFCKGDEacetylation[2]
379TVQWWFKKFCKGDESubiquitination[3]
382WWFKKFCKGDESLEDubiquitination[3]
485RIVTCDEKWILYDNRmethylation[4]
624LNNFLQGKRFHNQQDubiquitination[3]
661QLISRWQKCVDCNGSubiquitination[3]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] The SET and transposase domain protein Metnase enhances chromosome decatenation: regulation by automethylation.
 Williamson EA, Rasila KK, Corwin LK, Wray J, Beck BD, Severns V, Mobarak C, Lee SH, Nickoloff JA, Hromas R.
 Nucleic Acids Res. 2008 Oct;36(18):5822-31. [PMID: 18790802] 
Functional Description
 Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA. 
Sequence Annotation
 DOMAIN 60 123 Pre-SET.
 DOMAIN 126 250 SET.
 DOMAIN 270 286 Post-SET.
 DNA_BIND 351 382 H-T-H motif (By similarity).
 DNA_BIND 415 435 H-T-H motif.
 REGION 1 332 Histone-lysine N-methyltransferase.
 REGION 333 671 Mariner transposase Hsmar1.
 METAL 483 483 Magnesium.
 METAL 575 575 Magnesium.
 MOD_RES 495 495 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Chromatin regulator; Chromosome; Coiled coil; Complete proteome; DNA damage; DNA repair; DNA-binding; Endonuclease; Hydrolase; Magnesium; Metal-binding; Methyltransferase; Multifunctional enzyme; Nuclease; Nucleus; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 671 AA 
Protein Sequence
MAEFKEKPEA PTEQLDVACG QENLPVGAWP PGAAPAPFQY TPDHVVGPGA DIDPTQITFP 60
GCICVKTPCL PGTCSCLRHG ENYDDNSCLR DIGSGGKYAE PVFECNVLCR CSDHCRNRVV 120
QKGLQFHFQV FKTHKKGWGL RTLEFIPKGR FVCEYAGEVL GFSEVQRRIH LQTKSDSNYI 180
IAIREHVYNG QVMETFVDPT YIGNIGRFLN HSCEPNLLMI PVRIDSMVPK LALFAAKDIV 240
PEEELSYDYS GRYLNLTVSE DKERLDHGKL RKPCYCGAKS CTAFLPFDSS LYCPVEKSNI 300
SCGNEKEPSM CGSAPSVFPS CKRLTLETMK MMLDKKQIRA IFLFEFKMGR KAAETTRNIN 360
NAFGPGTANE RTVQWWFKKF CKGDESLEDE ERSGRPSEVD NDQLRAIIEA DPLTTTREVA 420
EELNVNHSTV VRHLKQIGKV KKLDKWVPHE LTENQKNRRF EVSSSLILRN HNEPFLDRIV 480
TCDEKWILYD NRRRSAQWLD QEEAPKHFPK PILHPKKVMV TIWWSAAGLI HYSFLNPGET 540
ITSEKYAQEI DEMNQKLQRL QLALVNRKGP ILLHDNARPH VAQPTLQKLN ELGYEVLPHP 600
PYSPDLLPTN YHVFKHLNNF LQGKRFHNQQ DAENAFQEFV ESQSTDFYAT GINQLISRWQ 660
KCVDCNGSYF D 671 
Gene Ontology
 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IC:UniProtKB.
 GO:0004519; F:endonuclease activity; IDA:UniProtKB.
 GO:0018024; F:histone-lysine N-methyltransferase activity; IEA:EC.
 GO:0043566; F:structure-specific DNA binding; IDA:UniProtKB.
 GO:0004803; F:transposase activity; TAS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000737; P:DNA catabolic process, endonucleolytic; IDA:UniProtKB.
 GO:0000729; P:DNA double-strand break processing; IDA:UniProtKB.
 GO:0015074; P:DNA integration; IEA:InterPro.
 GO:0071157; P:negative regulation of cell cycle arrest; IMP:UniProtKB.
 GO:2001251; P:negative regulation of chromosome organization; IDA:UniProtKB.
 GO:2001034; P:positive regulation of double-strand break repair via nonhomologous end joining; IDA:UniProtKB.
 GO:0006313; P:transposition, DNA-mediated; TAS:UniProtKB. 
Interpro
 IPR003616; Post-SET_dom.
 IPR007728; Pre-SET_dom.
 IPR003606; Pre-SET_Zn-bd_sub.
 IPR001214; SET_dom.
 IPR001888; Transposase_1.
 IPR002492; Transposase_Tc1-like. 
Pfam
 PF01498; HTH_Tnp_Tc3_2
 PF05033; Pre-SET
 PF00856; SET
 PF01359; Transposase_1 
SMART
 SM00508; PostSET
 SM00468; PreSET
 SM00317; SET 
PROSITE
 PS50868; POST_SET
 PS50867; PRE_SET
 PS50280; SET 
PRINTS