| Tag | Content |
|---|
| CPLM ID | CPLM-019859 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Kelch-like protein 22 |
Protein Synonyms/Alias | |
Gene Name | Klhl22 |
Gene Synonyms/Alias | Kelchl |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 260 | VLQRLHDKLGPSPLR | ubiquitination | [1] | | 433 | WDYVAPLKKEVYAHA | ubiquitination | [1] | | 434 | DYVAPLKKEVYAHAG | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (By similarity). |
Sequence Annotation | DOMAIN 50 117 BTB.
REPEAT 299 349 Kelch 1.
REPEAT 350 399 Kelch 2.
REPEAT 400 446 Kelch 3.
REPEAT 448 493 Kelch 4.
REPEAT 495 544 Kelch 5.
REPEAT 545 593 Kelch 6.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 463 463 Phosphothreonine (By similarity).
MOD_RES 466 466 Phosphotyrosine (By similarity).
MOD_RES 475 475 Phosphothreonine (By similarity). |
Keyword | Acetylation; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Kelch repeat; Mitosis; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 634 AA |
Protein Sequence | MAEEQDFAQL CRLPTQPSHS HCVNNTYRST QHSQALLRGL LALRDSGILF DVVLVVEGKH 60
IEAHRILLAA SCDYFRGMFA GGLKEMEQEE VLIHGVSYNA MCQILHFIYT SELELSLSNV 120
QETLVAACQL QIPEIIHFCC DFLMSWVDEE NILDVYRLAD LFDLNHLTQQ LDTYILKNFV 180
AFSRTDKYRQ LPLEKVYSLL SSNRLEVSCE TEVYEGALLY HYSLEQVQAD QISLNEPPKL 240
LETVRFPLME AEVLQRLHDK LGPSPLRDTV ASALMYHRNE ILQPSLQGPQ TELRSDFQCV 300
VGFGGIHSTP STILSDQAKY LNPLLGEWKH FTASLAPRMS NQGIAVLNNF VYLIGGDNNV 360
QGFRAESRCW RYDPRHNRWF QIQSLQQEHA DLCVCVVGKY IYAVAGRDYH NDLSAVERYD 420
PATNSWDYVA PLKKEVYAHA GTTLQGKMYI TCGRRGEDYL KETHCYDPGS NTWHTLADGP 480
VRRAWHGMAA LLDKLFVIGG SNNDAGYRRD VHQVACYSCT SRQWSSVCPL PAGHGEPGIA 540
VLDSRIYVLG GRSHNRGSRT GYVHIYDMEK DCWEEGPQLN NSISGLAACV LTLPRSLLHE 600
QPRGTPNRSQ ADADFASEVM SVSDWEEFDN SSED 634 |
Gene Ontology | GO:0005813; C:centrosome; ISS:UniProtKB. GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB. GO:0005737; C:cytoplasm; ISS:UniProtKB. GO:0072686; C:mitotic spindle; ISS:UniProtKB. GO:0005827; C:polar microtubule; ISS:UniProtKB. GO:0051301; P:cell division; ISS:UniProtKB. GO:0000070; P:mitotic sister chromatid segregation; ISS:UniProtKB. GO:0007094; P:mitotic spindle assembly checkpoint; ISS:UniProtKB. GO:0006513; P:protein monoubiquitination; ISS:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |