CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001018
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DnaJ homolog subfamily A member 2 
Protein Synonyms/Alias
 Cell cycle progression restoration gene 3 protein; Dnj3; Dj3; HIRA-interacting protein 4; Renal carcinoma antigen NY-REN-14 
Gene Name
 DNAJA2 
Gene Synonyms/Alias
 CPR3; HIRIP4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
25GASENELKKAYRKLAubiquitination[1, 2, 3]
26ASENELKKAYRKLAKubiquitination[1, 3]
39AKEYHPDKNPNAGDKubiquitination[2, 4, 5]
46KNPNAGDKFKEISFAubiquitination[1, 3, 5]
62EVLSNPEKRELYDRYubiquitination[2, 4, 5, 6]
131LEDLYNGKTTKLQLSubiquitination[1, 3, 4]
134LYNGKTTKLQLSKNVubiquitination[1, 2, 3, 4, 7]
139TTKLQLSKNVLCSACubiquitination[4]
152ACSGQGGKSGAVQKCubiquitination[2, 4, 7, 8, 9]
158GKSGAVQKCSACRGRubiquitination[2, 6, 7]
199EGEVINEKDRCKKCEubiquitination[2, 4, 7]
302VVKYPPGKVIEPGCVubiquitination[4]
326QYRNPFEKGDLYIKFubiquitination[1, 3, 5, 9]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [9] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Co-chaperone of Hsc70. 
Sequence Annotation
 DOMAIN 8 70 J.
 REPEAT 143 150 CXXCXGXG motif.
 REPEAT 159 166 CXXCXGXG motif.
 REPEAT 186 193 CXXCXGXG motif.
 REPEAT 202 209 CXXCXGXG motif.
 ZN_FING 130 214 CR-type.
 METAL 143 143 Zinc 1 (By similarity).
 METAL 146 146 Zinc 1 (By similarity).
 METAL 159 159 Zinc 2 (By similarity).
 METAL 162 162 Zinc 2 (By similarity).
 METAL 186 186 Zinc 2 (By similarity).
 METAL 189 189 Zinc 2 (By similarity).
 METAL 202 202 Zinc 1 (By similarity).
 METAL 205 205 Zinc 1 (By similarity).
 MOD_RES 78 78 Phosphoserine.
 MOD_RES 409 409 Cysteine methyl ester (Probable).
 LIPID 409 409 S-farnesyl cysteine.  
Keyword
 Chaperone; Complete proteome; Lipoprotein; Membrane; Metal-binding; Methylation; Phosphoprotein; Prenylation; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 412 AA 
Protein Sequence
MANVADTKLY DILGVPPGAS ENELKKAYRK LAKEYHPDKN PNAGDKFKEI SFAYEVLSNP 60
EKRELYDRYG EQGLREGSGG GGGMDDIFSH IFGGGLFGFM GNQSRSRNGR RRGEDMMHPL 120
KVSLEDLYNG KTTKLQLSKN VLCSACSGQG GKSGAVQKCS ACRGRGVRIM IRQLAPGMVQ 180
QMQSVCSDCN GEGEVINEKD RCKKCEGKKV IKEVKILEVH VDKGMKHGQR ITFTGEADQA 240
PGVEPGDIVL LLQEKEHEVF QRDGNDLHMT YKIGLVEALC GFQFTFKHLD GRQIVVKYPP 300
GKVIEPGCVR VVRGEGMPQY RNPFEKGDLY IKFDVQFPEN NWINPDKLSE LEDLLPSRPE 360
VPNIIGETEE VELQEFDSTR GSGGGQRREA YNDSSDEESS SHHGPGVQCA HQ 412 
Gene Ontology
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
 GO:0006457; P:protein folding; IEA:InterPro.
 GO:0009408; P:response to heat; IEA:InterPro. 
Interpro
 IPR012724; DnaJ.
 IPR002939; DnaJ_C.
 IPR001623; DnaJ_domain.
 IPR018253; DnaJ_domain_CS.
 IPR008971; HSP40/DnaJ_pept-bd.
 IPR001305; HSP_DnaJ_Cys-rich_dom. 
Pfam
 PF00226; DnaJ
 PF01556; DnaJ_C
 PF00684; DnaJ_CXXCXGXG 
SMART
 SM00271; DnaJ 
PROSITE
 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS51188; ZF_CR 
PRINTS
 PR00625; JDOMAIN.