CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018764
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mediator of RNA polymerase II transcription subunit 1 
Protein Synonyms/Alias
 Mediator complex subunit 1; Peroxisome proliferator-activated receptor-binding protein; PBP; PPAR-binding protein; Thyroid hormone receptor-associated protein complex 220 kDa component; Trap220; Thyroid receptor-interacting protein 2; TR-interacting protein 2; TRIP-2 
Gene Name
 Med1 
Gene Synonyms/Alias
 Crsp210; Drip205; Pbp; Pparbp; Trap220; Trip2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
202TNAAPLDKILHGSVGubiquitination[1]
1076KGTVMVGKPSSHSQYacetylation[2]
1212DKLASPMKPVPGTPPacetylation[2, 3]
1311SLTAVIDKLKHGVVTacetylation[4]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Essential for embryogenesis, including development of the central nervous system, heart, liver and placenta and for erythropoiesis. Also required for normal transcriptional control of thyroid-stimulating hormone beta (TSHB) in the pituitary. 
Sequence Annotation
 REGION 1 670 Interaction with the Mediator complex and
 REGION 16 590 Interaction with ESR1 (By similarity).
 REGION 108 212 Interaction with the Mediator complex (By
 REGION 215 390 Interaction with the Mediator complex (By
 REGION 405 644 Interaction with THRA (By similarity).
 REGION 542 789 Interaction with VDR (By similarity).
 REGION 622 701 Interaction with PPARGC1A and THRA (By
 REGION 637 716 Interaction with GATA1.
 REGION 656 1066 Interaction with ESR1 (By similarity).
 REGION 1251 1423 Interaction with TP53 (By similarity).
 MOTIF 604 608 LXXLL motif 1.
 MOTIF 645 649 LXXLL motif 2.
 MOD_RES 664 664 Phosphoserine (By similarity).
 MOD_RES 795 795 Phosphoserine (By similarity).
 MOD_RES 805 805 Phosphothreonine (By similarity).
 MOD_RES 953 953 Phosphoserine.
 MOD_RES 955 955 Phosphoserine.
 MOD_RES 1032 1032 Phosphothreonine; by MAPK1 or MAPK3 (By
 MOD_RES 1051 1051 Phosphothreonine (By similarity).
 MOD_RES 1057 1057 Phosphothreonine (By similarity).
 MOD_RES 1158 1158 Phosphoserine (By similarity).
 MOD_RES 1179 1179 N6-acetyllysine (By similarity).
 MOD_RES 1209 1209 Phosphoserine (By similarity).
 MOD_RES 1217 1217 Phosphothreonine (By similarity).
 MOD_RES 1225 1225 Phosphoserine (By similarity).
 MOD_RES 1349 1349 Phosphoserine (By similarity).
 MOD_RES 1405 1405 Phosphoserine (By similarity).
 MOD_RES 1435 1435 Phosphoserine (By similarity).
 MOD_RES 1459 1459 Phosphothreonine; by MAPK1 or MAPK3 (By
 MOD_RES 1465 1465 Phosphoserine (By similarity).
 MOD_RES 1481 1481 Phosphoserine (By similarity).
 MOD_RES 1483 1483 Phosphoserine (By similarity).
 MOD_RES 1484 1484 Phosphoserine (By similarity).
 MOD_RES 1523 1523 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Activator; Alternative splicing; Complete proteome; Developmental protein; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1575 AA 
Protein Sequence
MKAQGETEDS ERLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS 60
CLETLQKALK VTSLPAMTDR LESIARQNGL GSHLSASGTE CYITSDMFYV EVQLDPAGQL 120
CDVKVAHHGE NPVSCPELVQ QLREKNFEEF SKHLKGLVNL YNLPGDNKLK TKMYLALQSL 180
EQDLSKMAIM YWKATNAAPL DKILHGSVGY LTPRSGGHLM NMKYYASPSD LLDDKTASPI 240
ILHEKNVPRS LGMNASVTIE GTSAMYKLPI APLIMGSHPA DNKWTPSFSA VTSANSVDLP 300
ACFFLKFPQP IPVSKAFVQK LQNCTGIPLF ETPPTYLPLY ELITQFELSK DPDPLPLNHN 360
MRFYAALPGQ QHCYFLNKDA PLPDGQSLQG TLVSKITFQH PGRVPLILNM IRHQVAYNTL 420
IGSCVKRTIL KEDSPGLLQF EVCPLSESRF SVSFQHPVND SLVCVVMDVQ DSTHVSCKLY 480
KGLSDALICT DDFIAKVVQR CMSIPVTMRA IRRKAETIQA DTPALSLIAE TVEDMVKKNL 540
PPASSPGYGM TTGNNPMSGT TTPTNTFPGG PITTLFNMSM SIKDRHESVG HGEDFSKVSQ 600
NPILTSLLQI TGNGGSTIGS SPTPPHHTPP PVSSMAGNTK NHPMLMNLLK DNPAQDFSTL 660
YGSSPLERQN SSSGSPRMEM CSGSNKAKKK KSSRVPPDKP KHQTEDDFQR ELFSMDVDSQ 720
NPMFDVSMTA DALDTPHITP APSQCSTPPA TYPQPVSHPQ PSIQRMVRLS SSDSIGPDVT 780
DILSDIAEEA SKLPSTSDDC PPIGTPVRDS SSSGHSQSAL FDSDVFQTNN NENPYTDPAD 840
LIADAAGSPN SDSPTNHFFP DGVDFNPDLL NSQSQSGFGE EYFDESSQSG DNDDFKGFAS 900
QALNTLGMPM LGGDNGEPKF KGSSQADTVD FSIISVAGKA LGAADLMEHH SGSQSPLLTT 960
GELGKEKTQK RVKEGNGTGA SSGSGPGSDS KPGKRSRTPS NDGKSKDKPP KRKKADTEGK 1020
SPSHSSSNRP FTPPTSTGGS KSPGSSGRSQ TPPGVATPPI PKITIQIPKG TVMVGKPSSH 1080
SQYTSSGSVS SSGSKSHHSH SSSSSSLASA STSGKVKSSK SEGSSSSKLS GSMYASQGSS 1140
GSSQSKNSSQ TGGKPGSSPI TKHGLSSGSS STKMKPQGKP SSLMNPSISK PNISPSHSRP 1200
PGGSDKLASP MKPVPGTPPS SKAKSPISSG SSGSHVSGTS SSSGMKSSSG SASSGSVSQK 1260
TPPASNSCTP SSSSFSSSGS SMSSSQNQHG SSKGKSPSRN KKPSLTAVID KLKHGVVTSG 1320
PGGEDPIDSQ MGASTNSSNH PMSSKHNTSG GEFQSKREKS DKDKSKVSAS GGSVDSSKKT 1380
SESKNVGSTG VAKIIISKHD GGSPSIKAKV TLQKPGESGG DGLRPQIASS KNYGSPLISG 1440
STPKHERGSP SHSKSPAYTP QNVDSESESG SSIAERSYQN SPSSEDGIRP LPEYSTEKHK 1500
KHKKEKKKVR DKDRDKKKSH SMKPENWSKS PISSDPTASV TNNPILSADR PSRLSPDFMI 1560
GEEDDDLMDV ALIGN 1575 
Gene Ontology
 GO:0016592; C:mediator complex; IDA:MGI.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0001047; F:core promoter binding; ISS:UniProtKB.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; ISS:UniProtKB.
 GO:0032403; F:protein complex binding; IDA:MGI.
 GO:0004872; F:receptor activity; ISS:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; ISS:UniProtKB.
 GO:0000981; F:sequence-specific DNA binding RNA polymerase II transcription factor activity; IMP:UniProtKB.
 GO:0046966; F:thyroid hormone receptor binding; ISS:UniProtKB.
 GO:0030375; F:thyroid hormone receptor coactivator activity; IEA:Compara.
 GO:0006702; P:androgen biosynthetic process; ISS:UniProtKB.
 GO:0030521; P:androgen receptor signaling pathway; ISS:UniProtKB.
 GO:0001525; P:angiogenesis; IMP:BHF-UCL.
 GO:0007420; P:brain development; IMP:MGI.
 GO:0043010; P:camera-type eye development; IMP:MGI.
 GO:0000902; P:cell morphogenesis; ISS:UniProtKB.
 GO:0071364; P:cellular response to epidermal growth factor stimulus; ISS:UniProtKB.
 GO:0035729; P:cellular response to hepatocyte growth factor stimulus; IMP:MGI.
 GO:0071383; P:cellular response to steroid hormone stimulus; IEA:Compara.
 GO:0097067; P:cellular response to thyroid hormone stimulus; IEA:Compara.
 GO:0006260; P:DNA replication; IMP:MGI.
 GO:0035050; P:embryonic heart tube development; IMP:MGI.
 GO:0035162; P:embryonic hemopoiesis; IMP:MGI.
 GO:0035116; P:embryonic hindlimb morphogenesis; IMP:MGI.
 GO:0001892; P:embryonic placenta development; IMP:MGI.
 GO:0048822; P:enucleate erythrocyte development; IMP:MGI.
 GO:0060750; P:epithelial cell proliferation involved in mammary gland duct elongation; IMP:MGI.
 GO:0070371; P:ERK1 and ERK2 cascade; IEA:Compara.
 GO:0048821; P:erythrocyte development; IMP:BHF-UCL.
 GO:0045444; P:fat cell differentiation; IEA:Compara.
 GO:0030216; P:keratinocyte differentiation; ISS:UniProtKB.
 GO:0007595; P:lactation; IMP:MGI.
 GO:0002088; P:lens development in camera-type eye; IMP:BHF-UCL.
 GO:0001889; P:liver development; IMP:MGI.
 GO:0060745; P:mammary gland branching involved in pregnancy; IMP:MGI.
 GO:0060744; P:mammary gland branching involved in thelarche; IMP:MGI.
 GO:0035855; P:megakaryocyte development; IMP:BHF-UCL.
 GO:0030224; P:monocyte differentiation; IMP:MGI.
 GO:0042789; P:mRNA transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0010839; P:negative regulation of keratinocyte proliferation; ISS:UniProtKB.
 GO:0045665; P:negative regulation of neuron differentiation; IMP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0031100; P:organ regeneration; IMP:MGI.
 GO:0035357; P:peroxisome proliferator activated receptor signaling pathway; IMP:MGI.
 GO:0070318; P:positive regulation of G0 to G1 transition; IMP:MGI.
 GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
 GO:2000347; P:positive regulation of hepatocyte proliferation; IMP:MGI.
 GO:0060335; P:positive regulation of interferon-gamma-mediated signaling pathway; IMP:MGI.
 GO:0033148; P:positive regulation of intracellular estrogen receptor signaling pathway; IMP:MGI.
 GO:0045618; P:positive regulation of keratinocyte differentiation; ISS:UniProtKB.
 GO:0033601; P:positive regulation of mammary gland epithelial cell proliferation; IMP:MGI.
 GO:0033160; P:positive regulation of protein import into nucleus, translocation; IMP:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0006356; P:regulation of transcription from RNA polymerase I promoter; ISS:UniProtKB.
 GO:0070562; P:regulation of vitamin D receptor signaling pathway; IMP:MGI.
 GO:0003406; P:retinal pigment epithelium development; IMP:BHF-UCL.
 GO:0006590; P:thyroid hormone generation; IMP:MGI.
 GO:0002154; P:thyroid hormone mediated signaling pathway; ISS:UniProtKB.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0003222; P:ventricular trabecula myocardium morphogenesis; IMP:BHF-UCL. 
Interpro
 IPR019680; Mediator_Med1_met/fun. 
Pfam
 PF10744; Med1 
SMART
  
PROSITE
  
PRINTS