CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004279
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Junction plakoglobin 
Protein Synonyms/Alias
 Catenin gamma; Desmoplakin III; Desmoplakin-3 
Gene Name
 JUP 
Gene Synonyms/Alias
 CTNNG; DP3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
40CVPSVSSKGIMEEDEubiquitination[1]
124AEPSQLLKSAIVHLIubiquitination[1, 2]
149RALPELTKLLNDEDPubiquitination[1, 2]
161EDPVVVTKAAMIVNQubiquitination[1, 2]
171MIVNQLSKKEASRRAubiquitination[3]
224EGLLAIFKSGGIPALubiquitination[1, 2, 4]
326MRNYSYEKLLWTTSRubiquitination[2]
336WTTSRVLKVLSVCPSubiquitination[5]
345LSVCPSNKPAIVEAGubiquitination[5]
385NLSDVATKQEGLESVubiquitination[1, 2]
426TCNNSKNKTLVTQNSubiquitination[2]
448AILRAGDKDDITEPAubiquitination[3]
499PNQWPLVKATIGLIRubiquitination[1, 2, 5]
533RLVQLLVKAHQDAQRubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). 
Sequence Annotation
 REPEAT 132 171 ARM 1.
 REPEAT 172 215 ARM 2.
 REPEAT 216 255 ARM 3.
 REPEAT 258 297 ARM 4.
 REPEAT 298 341 ARM 5.
 REPEAT 342 381 ARM 6.
 REPEAT 383 420 ARM 7.
 REPEAT 423 464 ARM 8.
 REPEAT 470 510 ARM 9.
 REPEAT 512 551 ARM 10.
 REPEAT 574 613 ARM 11.
 REPEAT 615 661 ARM 12.
 REGION 132 297 Interaction with DSC1 and DSG1.
 REGION 574 661 Interaction with DSC1.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 182 182 Phosphoserine.
 MOD_RES 665 665 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Cardiomyopathy; Cell adhesion; Cell junction; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Disease mutation; Membrane; Palmoplantar keratoderma; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 745 AA 
Protein Sequence
MEVMNLMEQP IKVTEWQQTY TYDSGIHSGA NTCVPSVSSK GIMEEDEACG RQYTLKKTTT 60
YTQGVPPSQG DLEYQMSTTA RAKRVREAMC PGVSGEDSSL LLATQVEGQA TNLQRLAEPS 120
QLLKSAIVHL INYQDDAELA TRALPELTKL LNDEDPVVVT KAAMIVNQLS KKEASRRALM 180
GSPQLVAAVV RTMQNTSDLD TARCTTSILH NLSHHREGLL AIFKSGGIPA LVRMLSSPVE 240
SVLFYAITTL HNLLLYQEGA KMAVRLADGL QKMVPLLNKN NPKFLAITTD CLQLLAYGNQ 300
ESKLIILANG GPQALVQIMR NYSYEKLLWT TSRVLKVLSV CPSNKPAIVE AGGMQALGKH 360
LTSNSPRLVQ NCLWTLRNLS DVATKQEGLE SVLKILVNQL SVDDVNVLTC ATGTLSNLTC 420
NNSKNKTLVT QNSGVEALIH AILRAGDKDD ITEPAVCALR HLTSRHPEAE MAQNSVRLNY 480
GIPAIVKLLN QPNQWPLVKA TIGLIRNLAL CPANHAPLQE AAVIPRLVQL LVKAHQDAQR 540
HVAAGTQQPY TDGVRMEEIV EGCTGALHIL ARDPMNRMEI FRLNTIPLFV QLLYSSVENI 600
QRVAAGVLCE LAQDKEAADA IDAEGASAPL MELLHSRNEG TATYAAAVLF RISEDKNPDY 660
RKRVSVELTN SLFKHDPAAW EAAQSMIPIN EPYGDDMDAT YRPMYSSDVP LDPLEMHMDM 720
DGDYPIDTYS DGLRPPYPTA DHMLA 745 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IBA:RefGenome.
 GO:0016327; C:apicolateral plasma membrane; IEA:Compara.
 GO:0016323; C:basolateral plasma membrane; IBA:RefGenome.
 GO:0016342; C:catenin complex; IDA:BHF-UCL.
 GO:0005829; C:cytosol; ISS:BHF-UCL.
 GO:0030057; C:desmosome; IDA:BHF-UCL.
 GO:0005916; C:fascia adherens; IBA:RefGenome.
 GO:0071665; C:gamma-catenin-TCF7L2 complex; IDA:BHF-UCL.
 GO:0005882; C:intermediate filament; IEA:Compara.
 GO:0009898; C:internal side of plasma membrane; ISS:BHF-UCL.
 GO:0016328; C:lateral plasma membrane; IEA:Compara.
 GO:0032993; C:protein-DNA complex; IDA:BHF-UCL.
 GO:0030018; C:Z disc; IBA:RefGenome.
 GO:0005915; C:zonula adherens; ISS:BHF-UCL.
 GO:0042803; F:protein homodimerization activity; ISS:BHF-UCL.
 GO:0019901; F:protein kinase binding; IBA:RefGenome.
 GO:0005199; F:structural constituent of cell wall; IC:BHF-UCL.
 GO:0003713; F:transcription coactivator activity; IDA:BHF-UCL.
 GO:0034332; P:adherens junction organization; TAS:Reactome.
 GO:0003181; P:atrioventricular valve morphogenesis; IBA:RefGenome.
 GO:0086069; P:bundle of His cell to Purkinje myocyte communication; IMP:BHF-UCL.
 GO:0016477; P:cell migration; IMP:BHF-UCL.
 GO:0000902; P:cell morphogenesis; IBA:RefGenome.
 GO:0071681; P:cellular response to indole-3-methanol; IDA:UniProtKB.
 GO:0007016; P:cytoskeletal anchoring at plasma membrane; NAS:BHF-UCL.
 GO:0002159; P:desmosome assembly; IDA:BHF-UCL.
 GO:0050982; P:detection of mechanical stimulus; IDA:BHF-UCL.
 GO:0007398; P:ectoderm development; IBA:RefGenome.
 GO:0071603; P:endothelial cell-cell adhesion; ISS:BHF-UCL.
 GO:0007369; P:gastrulation; IBA:RefGenome.
 GO:0016331; P:morphogenesis of embryonic epithelium; IBA:RefGenome.
 GO:0003136; P:negative regulation of heart induction by canonical Wnt receptor signaling pathway; IBA:RefGenome.
 GO:0003308; P:negative regulation of Wnt receptor signaling pathway involved in heart development; IBA:RefGenome.
 GO:0007399; P:nervous system development; IBA:RefGenome.
 GO:0048599; P:oocyte development; IBA:RefGenome.
 GO:0090263; P:positive regulation of canonical Wnt receptor signaling pathway; IC:BHF-UCL.
 GO:0042307; P:positive regulation of protein import into nucleus; IDA:BHF-UCL.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0042127; P:regulation of cell proliferation; IDA:BHF-UCL.
 GO:0086091; P:regulation of heart rate by cardiac conduction; IMP:BHF-UCL.
 GO:0086005; P:regulation of ventricular cardiac muscle cell action potential; IMP:BHF-UCL.
 GO:0043588; P:skin development; IBA:RefGenome. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR013284; Beta-catenin. 
Pfam
 PF00514; Arm 
SMART
 SM00185; ARM 
PROSITE
 PS50176; ARM_REPEAT 
PRINTS
 PR01869; BCATNINFAMLY.