CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001736
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alcohol dehydrogenase 1 
Protein Synonyms/Alias
 Alcohol dehydrogenase I; YADH-1 
Gene Name
 ADH1 
Gene Synonyms/Alias
 ADC1; YOL086C; O0947 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
8MSIPETQKGVIFYESacetylation[1]
18IFYESHGKLEYKDIPacetylation[1]
22SHGKLEYKDIPVPKPacetylation[1]
28YKDIPVPKPKANELLubiquitination[2]
30DIPVPKPKANELLINacetylation[1]
39NELLINVKYSGVCHTubiquitination[2]
60GDWPLPVKLPLVGGHubiquitination[2]
81VGMGENVKGWKIGDYacetylation[1]
81VGMGENVKGWKIGDYubiquitination[2]
84GENVKGWKIGDYAGIacetylation[1]
84GENVKGWKIGDYAGIubiquitination[2]
164ITVYKALKSANLMAGubiquitination[2]
192SLAVQYAKAMGYRVLubiquitination[2]
207GIDGGEGKEELFRSIacetylation[1]
207GIDGGEGKEELFRSIubiquitination[2, 3]
224EVFIDFTKEKDIVGAacetylation[1]
226FIDFTKEKDIVGAVLacetylation[1, 4]
226FIDFTKEKDIVGAVLubiquitination[3]
234DIVGAVLKATDGGAHubiquitination[3]
276VGMPAGAKCCSDVFNubiquitination[2, 3]
287DVFNQVVKSISIVGSubiquitination[2, 3]
315FFARGLVKSPIKVVGubiquitination[2]
319GLVKSPIKVVGLSTLacetylation[1]
319GLVKSPIKVVGLSTLubiquitination[2, 3]
332TLPEIYEKMEKGQIVacetylation[1]
332TLPEIYEKMEKGQIVubiquitination[2, 3]
335EIYEKMEKGQIVGRYacetylation[1]
335EIYEKMEKGQIVGRYubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 
Functional Description
 This isozyme preferentially catalyzes the conversion of primary unbranched alcohols to their corresponding aldehydes. Also also shows activity toward secondary alcohols. 
Sequence Annotation
 NP_BIND 178 184 NAD (By similarity).
 NP_BIND 269 271 NAD (By similarity).
 METAL 44 44 Zinc 1; catalytic.
 METAL 67 67 Zinc 1; catalytic.
 METAL 98 98 Zinc 2.
 METAL 101 101 Zinc 2.
 METAL 104 104 Zinc 2.
 METAL 112 112 Zinc 2.
 METAL 154 154 Zinc 1; catalytic.
 BINDING 202 202 NAD (By similarity).
 BINDING 207 207 NAD (By similarity).
 BINDING 341 341 NAD (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 213 213 Phosphoserine.
 MOD_RES 223 223 Phosphothreonine.
 MOD_RES 279 279 Phosphoserine.
 MOD_RES 316 316 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Metal-binding; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 348 AA 
Protein Sequence
MSIPETQKGV IFYESHGKLE YKDIPVPKPK ANELLINVKY SGVCHTDLHA WHGDWPLPVK 60
LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA CEYCELGNES NCPHADLSGY 120
THDGSFQQYA TADAVQAAHI PQGTDLAQVA PILCAGITVY KALKSANLMA GHWVAISGAA 180
GGLGSLAVQY AKAMGYRVLG IDGGEGKEEL FRSIGGEVFI DFTKEKDIVG AVLKATDGGA 240
HGVINVSVSE AAIEASTRYV RANGTTVLVG MPAGAKCCSD VFNQVVKSIS IVGSYVGNRA 300
DTREALDFFA RGLVKSPIKV VGLSTLPEIY EKMEKGQIVG RYVVDTSK 348 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0004022; F:alcohol dehydrogenase (NAD) activity; IDA:SGD.
 GO:0019170; F:methylglyoxal reductase (NADH-dependent) activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000947; P:amino acid catabolic process to alcohol via Ehrlich pathway; IGI:SGD.
 GO:0043458; P:ethanol biosynthetic process involved in glucose fermentation to ethanol; IMP:SGD.
 GO:0006116; P:NADH oxidation; IDA:SGD. 
Interpro
 IPR013149; ADH_C.
 IPR013154; ADH_GroES-like.
 IPR002085; ADH_SF_Zn-type.
 IPR002328; ADH_Zn_CS.
 IPR011032; GroES-like.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF08240; ADH_N
 PF00107; ADH_zinc_N 
SMART
  
PROSITE
 PS00059; ADH_ZINC 
PRINTS