CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006840
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycine--tRNA ligase 1, mitochondrial 
Protein Synonyms/Alias
 Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase 1; GlyRS 1; GlyRS1 
Gene Name
 GRS1 
Gene Synonyms/Alias
 YBR121C; YBR0917 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
126DWMCRDLKTGEIFRAacetylation[1]
163EDANAAAKDDAEKKKacetylation[1]
189KLDDDVVKEYEEILAacetylation[1]
280FASASIGKSFRNEISacetylation[1]
280FASASIGKSFRNEISubiquitination[2]
321HPKFNEIKDIKLSFLacetylation[1]
324FNEIKDIKLSFLPRDacetylation[1]
343GSTEPIVKTVGEAVAacetylation[1]
343GSTEPIVKTVGEAVAubiquitination[2]
427YDLTVHSKKTKEKLVacetylation[1]
438EKLVVRQKLDNPIEVacetylation[1]
467KFRKDAPKVESHLLNacetylation[1]
483SQDDLASKAELLKANacetylation[1]
509GEVELDDKLVKIEQRacetylation[1]
601RKSQIPFKIDDSGVSacetylation[1]
611DSGVSIGKRYARNDEubiquitination[2]
657GSVENVIKAIRDITYacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs (By similarity). 
Sequence Annotation
 NP_BIND 283 285 ATP (By similarity).
 NP_BIND 293 298 ATP (By similarity).
 NP_BIND 410 411 ATP (By similarity).
 NP_BIND 535 538 ATP (By similarity).
 REGION 298 302 Substrate binding (By similarity).
 REGION 531 535 Substrate binding (By similarity).
 BINDING 132 132 Substrate (By similarity).
 BINDING 251 251 Substrate (By similarity).
 BINDING 388 388 Substrate (By similarity).
 BINDING 388 388 Substrate; via carbonyl oxygen (By
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 476 476 Phosphoserine.
 MOD_RES 528 528 Phosphoserine.
 MOD_RES 689 689 Phosphothreonine.  
Keyword
 Alternative initiation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 690 AA 
Protein Sequence
MSFFNISRRF YSQIVKKSVK IKRMSVEDIK KARAAVPFNR EQLESVLRGR FFYAPAFDLY 60
GGVSGLYDYG PPGCAFQNNI IDAWRKHFIL EEDMLEVDCT MLTPYEVLKT SGHVDKFSDW 120
MCRDLKTGEI FRADHLVEEV LEARLKGDQE ARGLVEDANA AAKDDAEKKK RKKKVKQIKA 180
VKLDDDVVKE YEEILAKIDG YSGPELGELM EKYDIGNPVT GETLESPRAF NLMFETAIGP 240
SGQLKGYLRP ETAQGQFLNF NKLLEFNNSK TPFASASIGK SFRNEISPRA GLLRVREFLM 300
AEIEHFVDPL DKSHPKFNEI KDIKLSFLPR DVQEAGSTEP IVKTVGEAVA SRMVDNETLG 360
YFIARIYQFL MKIGVDESKL RFRQHMANEM AHYAADCWDG ELKTSYGWIE CVGCADRSAY 420
DLTVHSKKTK EKLVVRQKLD NPIEVTKWEI DLTKKLFGPK FRKDAPKVES HLLNMSQDDL 480
ASKAELLKAN GKFTIKVDGV DGEVELDDKL VKIEQRTKVE HVREYVPSVI EPSFGIGRII 540
YSVFEHSFWN RPEDNARSVL SFPPLVAPTK VLLVPLSNHK DLVPVTTEVA KILRKSQIPF 600
KIDDSGVSIG KRYARNDELG TPFGVTIDFE SAKDHSVTLR ERDSTKQVRG SVENVIKAIR 660
DITYNGASWE EGTKDLTPFI AQAEAEAETD 690 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004820; F:glycine-tRNA ligase activity; ISS:UniProtKB.
 GO:0046983; F:protein dimerization activity; ISS:UniProtKB.
 GO:0015966; P:diadenosine tetraphosphate biosynthetic process; ISS:UniProtKB.
 GO:0006353; P:DNA-dependent transcription, termination; IMP:SGD.
 GO:0070150; P:mitochondrial glycyl-tRNA aminoacylation; IMP:SGD. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR027031; Gly-tRNA_synthase/POLG2.
 IPR002315; tRNA-synt_gly. 
Pfam
 PF03129; HGTP_anticodon
 PF00587; tRNA-synt_2b 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01043; TRNASYNTHGLY.