CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008452
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CDK-activating kinase assembly factor MAT1 
Protein Synonyms/Alias
 CDK7/cyclin-H assembly factor; Menage a trois; RING finger protein MAT1; p35; p36 
Gene Name
 Mnat1 
Gene Synonyms/Alias
 Mat1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
180QALKRKNKQAFLDELubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. 
Sequence Annotation
 REPEAT 142 161 UIM.
 ZN_FING 6 50 RING-type.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 51 51 Phosphothreonine (By similarity).
 MOD_RES 279 279 Phosphoserine (By similarity).  
Keyword
 Acetylation; Cell cycle; Complete proteome; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 309 AA 
Protein Sequence
MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV 60
QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE EVEEIVFNLT NNVDLENTKK 120
KMEIYQKENK DVIQKNKLKL TREQEELEEA LEVERQEHEQ RRLFIQKEEE LQQALKRKNK 180
QAFLDELESS DLPVALLLAQ HKDRSTQLEM QLEKPRSMKP VTFSTGIKMG QQISLAPIQK 240
LEEALYEYQP LQIETCGPQV PEQELLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA 300
FSGLFWQPR 309 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005675; C:holo TFIIH complex; ISS:UniProtKB.
 GO:0008094; F:DNA-dependent ATPase activity; IEA:Compara.
 GO:0008353; F:RNA polymerase II carboxy-terminal domain kinase activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007512; P:adult heart development; IMP:MGI.
 GO:0006915; P:apoptotic process; IMP:MGI.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
 GO:0044236; P:multicellular organismal metabolic process; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0050790; P:regulation of catalytic activity; IMP:MGI.
 GO:0006355; P:regulation of transcription, DNA-dependent; IMP:MGI.
 GO:0051592; P:response to calcium ion; IMP:MGI.
 GO:0006366; P:transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0021591; P:ventricular system development; IMP:MGI. 
Interpro
 IPR015877; Cdk-activating_kinase_MAT1_cen.
 IPR004575; MAT1/Tfb3.
 IPR003903; Ubiquitin-int_motif.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF06391; MAT1 
SMART
 SM00184; RING 
PROSITE
 PS50330; UIM
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS