| Tag | Content |
|---|
| CPLM ID | CPLM-007643 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | G protein-coupled receptor kinase 6 |
Protein Synonyms/Alias | G protein-coupled receptor kinase GRK6 |
Gene Name | GRK6 |
Gene Synonyms/Alias | GPRK6 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 139 | RLEQGPCKDLFQELT | ubiquitination | [1] | | 240 | NEKQILEKVNSRFVV | ubiquitination | [1] | | 313 | RIVYRDLKPENILLD | ubiquitination | [1] | | 343 | VPEGQTIKGRVGTVG | ubiquitination | [1, 2] | | 358 | YMAPEVVKNERYTFS | ubiquitination | [1] | | 402 | EEVERLVKEVPEEYS | ubiquitination | [1, 2] | | 426 | LCSQLLCKDPAERLG | ubiquitination | [1] | | 443 | GGSAREVKEHPLFKK | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA. Mol Cell Proteomics. 2012 May;11(5):148-59. [ PMID: 22505724] |
Functional Description | Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro). |
Sequence Annotation | DOMAIN 53 171 RGS.
DOMAIN 186 448 Protein kinase.
DOMAIN 449 514 AGC-kinase C-terminal.
NP_BIND 192 200 ATP.
NP_BIND 264 270 ATP.
NP_BIND 315 318 ATP.
REGION 1 185 N-terminal.
ACT_SITE 311 311 Proton acceptor (By similarity).
BINDING 215 215 ATP.
MOD_RES 484 484 Phosphoserine.
MOD_RES 485 485 Phosphothreonine.
LIPID 561 561 S-palmitoyl cysteine (Probable).
LIPID 562 562 S-palmitoyl cysteine (Probable).
LIPID 565 565 S-palmitoyl cysteine (Probable). |
Keyword | 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Kinase; Lipoprotein; Membrane; Nucleotide-binding; Palmitate; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Wnt signaling pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 576 AA |
Protein Sequence | MELENIVANT VLLKAREGGG GNRKGKSKKW RQMLQFPHIS QCEELRLSLE RDYHSLCERQ 60
PIGRLLFREF CATRPELSRC VAFLDGVAEY EVTPDDKRKA CGRQLTQNFL SHTGPDLIPE 120
VPRQLVTNCT QRLEQGPCKD LFQELTRLTH EYLSVAPFAD YLDSIYFNRF LQWKWLERQP 180
VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK 240
VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE 300
DLHRERIVYR DLKPENILLD DHGHIRISDL GLAVHVPEGQ TIKGRVGTVG YMAPEVVKNE 360
RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVPEEYSE RFSPQARSLC 420
SQLLCKDPAE RLGCRGGSAR EVKEHPLFKK LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI 480
EQFSTVKGVE LEPTDQDFYQ KFATGSVPIP WQNEMVETEC FQELNVFGLD GSVPPDLDWK 540
GQPPAPPKKG LLQRLFSRQR IAVETAATAR KSSPPASSPQ PEAPTSSWR 589 |
Gene Ontology | GO:0016020; C:membrane; IEA:UniProtKB-SubCell. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004703; F:G-protein coupled receptor kinase activity; IEA:EC. GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; TAS:ProtInc. GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro. GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |