CPLM-010545

Genbank Nucleotide ID

ADP-ribosylation factor 1

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
36	GKTTILYKLKLGEIV	ubiquitination	[1]
38	TTILYKLKLGEIVTT	ubiquitination	[1]
73	WDVGGQDKIRPLWRH	ubiquitination	[1, 2, 3]
127	VLLVFANKQDLPNAM	ubiquitination	[1, 4]
142	NAAEITDKLGLHSLR	ubiquitination	[1, 2, 3]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]

Functional Description

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.

NP_BIND 24 31 GTP (By similarity).
NP_BIND 126 129 GTP (By similarity).
MOD_RES 2 2 N-acetylglycine; alternate.
LIPID 2 2 N-myristoyl glycine; alternate.

3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; ER-Golgi transport; Golgi apparatus; GTP-binding; Lipoprotein; Myristate; Nucleotide-binding; Protein transport; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0000139; C:Golgi membrane; TAS:Reactome.
GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
GO:0005886; C:plasma membrane; TAS:ProtInc.
GO:0030017; C:sarcomere; IEA:Compara.
GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
GO:0003924; F:GTPase activity; TAS:ProtInc.
GO:0005057; F:receptor signaling protein activity; TAS:ProtInc.
GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
GO:0006878; P:cellular copper ion homeostasis; IMP:UniProtKB.
GO:0048205; P:COPI coating of Golgi vesicle; TAS:Reactome.
GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.
GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome.
GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; TAS:Reactome.
GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
GO:0016032; P:viral reproduction; TAS:Reactome.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR024156; Small_GTPase_ARF.
IPR006689; Small_GTPase_ARF/SAR.