| Tag | Content |
|---|
| CPLM ID | CPLM-021414 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | ATP-dependent DNA helicase PIF1 |
Protein Synonyms/Alias | PIF1/RRM3 DNA helicase-like protein |
Gene Name | PIF1 |
Gene Synonyms/Alias | C15orf20 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 204 | RLSLPSTKPQLSEEQ | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | DNA-dependent ATPase and DNA helicase inhibiting telomerase activity by unwinding DNA/RNA duplex formed by telomerase RNA and telomeric DNA in a 5' to 3' polarity. Negatively regulates telomere length and such inhibition requires its ATPase activity. Tightly cell cycle regulated and expressed in late S/G2 phase. |
Sequence Annotation | NP_BIND 228 235 ATP (Potential).
REGION 167 641 Hydrolyzes ATP in the presence of both
MOD_RES 27 27 Phosphoserine.
MOD_RES 151 151 Phosphoserine. |
Keyword | Alternative initiation; Alternative splicing; ATP-binding; Complete proteome; DNA-binding; Helicase; Hydrolase; Mitochondrion; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 641 AA |
Protein Sequence | MLSGIEAAAG EYEDSELRCR VAVEELSPGG QPRRRQALRT AELSLGRNER RELMLRLQAP 60
GPAGRPRCFP LRAARLFTRF AEAGRSTLRL PAHDTPGAGA VQLLLSDCPP DRLRRFLRTL 120
RLKLAAAPGP GPASARAQLL GPRPRDFVTI SPVQPEERRL RAATRVPDTT LVKRPVEPQA 180
GAEPSTEAPR WPLPVKRLSL PSTKPQLSEE QAAVLRAVLK GQSIFFTGSA GTGKSYLLKR 240
ILGSLPPTGT VATASTGVAA CHIGGTTLHA FAGIGSGQAP LAQCVALAQR PGVRQGWLNC 300
QRLVIDEISM VEADLFDKLE AVARAVRQQN KPFGGIQLII CGDFLQLPPV TKGSQPPRFC 360
FQSKSWKRCV PVTLELTKVW RQADQTFISL LQAVRLGRCS DEVTRQLQAT ASHKVGRDGI 420
VATRLCTHQD DVALTNERRL QELPGKVHRF EAMDSNPELA STLDAQCPVS QLLQLKLGAQ 480
VMLVKNLSVS RGLVNGARGV VVGFEAEGRG LPQVRFLCGV TEVIHADRWT VQATGGQLLS 540
RQQLPLQLAW AMSIHKSQGM TLDCVEISLG RVFASGQAYV ALSRARSLQG LRVLDFDPMA 600
VRCDPRVLHF YATLRRGRSL SLESPDDDEA ASDQENMDPI L 641 |
Gene Ontology | GO:0000784; C:nuclear chromosome, telomeric region; IDA:HGNC. GO:0005524; F:ATP binding; IDA:HGNC. GO:0043141; F:ATP-dependent 5'-3' DNA helicase activity; IDA:HGNC. GO:0033682; F:ATP-dependent 5'-3' DNA/RNA helicase activity; IDA:BHF-UCL. GO:0000287; F:magnesium ion binding; IDA:HGNC. GO:0017116; F:single-stranded DNA-dependent ATP-dependent DNA helicase activity; IDA:HGNC. GO:0042162; F:telomeric DNA binding; IDA:HGNC. GO:0051974; P:negative regulation of telomerase activity; IDA:HGNC. GO:0032204; P:regulation of telomere maintenance; IDA:HGNC. |
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