UniProt Accession

 FABA_ECOLI; P0A6Q3; P18391; Q59383 

Genbank Protein ID

 J03186; U00096; AP009048; U37057; U56977 

Genbank Nucleotide ID

 AAA96496.1; AAC74040.1; BAA35712.1; AAC44389.1; AAC44399.1 

Protein Name

 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase 

Protein Synonyms/Alias

 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA; Beta-hydroxydecanoyl thioester dehydrase; Trans-2-decenoyl-[acyl-carrier-protein] isomerase 

Gene Name

 fabA 

Gene Synonyms/Alias

 b0954; JW0937 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
10	DKRESYTKEDLLASG	acetylation	[1, 2]
25	RGELFGAKGPQLPAP	acetylation	[2]
42	LMMDRVVKMTETGGN	acetylation	[1, 2]
113	ALGVGEVKFTGQVLP	acetylation	[2]
123	GQVLPTAKKVTYRIH	acetylation	[2]
124	QVLPTAKKVTYRIHF	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl- ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)- decenoyl-ACP. Can catalyze the dehydratase reaction for beta- hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length. Is inactive in the dehydration of long chain unsaturated beta-hydroxyacyl-ACP. 

Sequence Annotation

 ACT_SITE 71 71  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Fatty acid biosynthesis; Fatty acid metabolism; Isomerase; Lipid biosynthesis; Lipid metabolism; Lyase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 172 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008693; F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:HAMAP.
 GO:0047451; F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; IEA:EC.
 GO:0034017; F:trans-2-decenoyl-acyl-carrier-protein isomerase activity; IEA:HAMAP.
 GO:0006633; P:fatty acid biosynthetic process; IGI:EcoliWiki. 

Interpro

 IPR010083; FabA.
 IPR013114; FabA_FabZ. 

Pfam

 PF07977; FabA 

SMART

  

PROSITE

  

PRINTS