CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015768
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein MCM10 homolog 
Protein Synonyms/Alias
 HsMCM10 
Gene Name
 MCM10 
Gene Synonyms/Alias
 PRO2249 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
110APAPRREKTNEELQEubiquitination[1]
134KALQEQLKVTTIKQTubiquitination[1]
139QLKVTTIKQTASPARubiquitination[1]
313PQSVNSGKTFSIWKLubiquitination[1, 2]
360ILNANPMKPKDGSEEubiquitination[1]
395GTCKAKKKNGEPCTQubiquitination[1]
481AAAVAPKKKIQTTLSubiquitination[1]
482AAVAPKKKIQTTLSNubiquitination[1]
520LSCSEEFKELMDLPTubiquitination[1]
566SALLKQQKQRMLEMRubiquitination[1]
627APATMTPKLGRGVLEubiquitination[1, 3, 4, 5]
658LSALAEAKKLAAITKubiquitination[1]
659SALAEAKKLAAITKLubiquitination[1]
665KKLAAITKLRAKGQVubiquitination[1]
695PQDILEVKERVEKNTubiquitination[1]
733LESEEFQKILKAKSKubiquitination[1]
763YFEPLVKKEQMEEKMubiquitination[1]
811YHWHDGVKRFFKCPCubiquitination[1]
815DGVKRFFKCPCGNRSubiquitination[1]
854LKEKTGPKIGGETLLubiquitination[1]
869PRGEEHAKFLNSLK*ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. 
Sequence Annotation
 REGION 1 156 N-terminal domain (By similarity).
 REGION 238 389 OB-fold domain (By similarity).
 REGION 390 415 Zinc finger-like 1.
 REGION 783 802 Zinc finger-like 2 (By similarity).
 REGION 816 836 Zinc finger-like 3 (By similarity).
 MOD_RES 85 85 Phosphothreonine.
 MOD_RES 93 93 Phosphoserine.
 CROSSLNK 627 627 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; DNA replication; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 875 AA 
Protein Sequence
MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG DGESYTEEAD 60
DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV LPAPAPRREK TNEELQEELR 120
NLQEQMKALQ EQLKVTTIKQ TASPARLQKS PVEKSPRPPL KERRVQRIQE STCFSAELDV 180
PALPRTKRVA RTPKASPPDP KSSSSRMTSA PSQPLQTISR NKPSGITRGQ IVGTPGSSGE 240
TTQPICVEAF SGLRLRRPRV SSTEMNKKMT GRKLIRLSQI KEKMAREKLE EIDWVTFGVI 300
LKKVTPQSVN SGKTFSIWKL NDLRDLTQCV SLFLFGEVHK ALWKTEQGTV VGILNANPMK 360
PKDGSEEVCL SIDHPQKVLI MGEALDLGTC KAKKKNGEPC TQTVNLRDCE YCQYHVQAQY 420
KKLSAKRADL QSTFSGGRIP KKFARRGTSL KERLCQDGFY YGGVSSASYA ASIAAAVAPK 480
KKIQTTLSNL VVKGTNLIIQ ETRQKLGIPQ KSLSCSEEFK ELMDLPTCGA RNLKQHLAKA 540
TASGIMGSPK PAIKSISASA LLKQQKQRML EMRRRKSEEI QKRFLQSSSE VESPAVPSSS 600
RQPPAQPPRT GSEFPRLEGA PATMTPKLGR GVLEGDDVLF YDESPPPRPK LSALAEAKKL 660
AAITKLRAKG QVLTKTNPNS IKKKQKDPQD ILEVKERVEK NTMFSSQAED ELEPARKKRR 720
EQLAYLESEE FQKILKAKSK HTGILKEAEA EMQERYFEPL VKKEQMEEKM RNIREVKCRV 780
VTCKTCAYTH FKLLETCVSE QHEYHWHDGV KRFFKCPCGN RSISLDRLPN KHCSNCGLYK 840
WERDGMLKEK TGPKIGGETL LPRGEEHAKF LNSLK 875 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; TAS:Reactome.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome. 
Interpro
 IPR015411; Rep_factor_Mcm10.
 IPR015408; Znf_Mcm10/DnaG. 
Pfam
 PF09332; Mcm10
 PF09329; zf-primase 
SMART
  
PROSITE
  
PRINTS