UniProt Accession

 PNKP_HUMAN; Q96T60; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0 

Genbank Protein ID

 AF126486; AF125807; AF120499; AF354258; AY133033; AC018766; BC033822 

Genbank Nucleotide ID

 AAD51135.1; AAD50639.1; AAD47379.1; AAK57340.1; AAM82170.1; AAF44716.1; AAH33822.1 

Protein Name

 Bifunctional polynucleotide phosphatase/kinase 

Protein Synonyms/Alias

 DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphatase; Polynucleotide 3'-phosphatase; 2'(3')-polynucleotidase; Polynucleotide 5'-hydroxyl-kinase 

Gene Name

 PNKP 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
162	VFTAAGVKPQGKVAG	ubiquitination	[1]
183	LITTRSGKVFPTGPS	ubiquitination	[1]
226	QMSIGRGKLPAEEFK	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. 

Sequence Annotation

 DOMAIN 6 110 FHA.
 NP_BIND 372 379 ATP (Potential).
 REGION 146 337 Phosphatase (By similarity).
 REGION 341 516 Kinase (By similarity).
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 118 118 Phosphothreonine.
 MOD_RES 122 122 Phosphothreonine.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Direct protein sequencing; Disease mutation; DNA damage; DNA repair; Hydrolase; Kinase; Multifunctional enzyme; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Primary microcephaly; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 521 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0046404; F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; IDA:UniProtKB.
 GO:0003684; F:damaged DNA binding; NAS:UniProtKB.
 GO:0003690; F:double-stranded DNA binding; TAS:UniProtKB.
 GO:0004519; F:endonuclease activity; NAS:UniProtKB.
 GO:0019201; F:nucleotide kinase activity; IDA:UniProtKB.
 GO:0046403; F:polynucleotide 3'-phosphatase activity; IDA:UniProtKB.
 GO:0042769; P:DNA damage response, detection of DNA damage; IDA:UniProtKB.
 GO:0006261; P:DNA-dependent DNA replication; NAS:UniProtKB.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; NAS:UniProtKB.
 GO:0006979; P:response to oxidative stress; IDA:UniProtKB.
 GO:0009314; P:response to radiation; NAS:UniProtKB. 

Interpro

 IPR000253; FHA_dom.
 IPR023214; HAD-like_dom.
 IPR006549; HAD-SF_hydro_IIIA.
 IPR027417; P-loop_NTPase.
 IPR013954; PNK3P.
 IPR015636; PNK_3Pase_euk.
 IPR006550; PNK_3Pase_met.
 IPR006551; Polynucleotide_phosphatase.
 IPR008984; SMAD_FHA_domain. 

Pfam

 PF08645; PNK3P 

SMART

  

PROSITE

 PS50006; FHA_DOMAIN 

PRINTS