UniProt Accession

 4F2_RAT; Q794F9 

Genbank Protein ID

 U59324; AB015433; BC061989 

Genbank Nucleotide ID

 AAC53560.1; BAA33036.1; AAH61989.1 

Protein Name

 4F2 cell-surface antigen heavy chain 

Protein Synonyms/Alias

 4F2hc; CD98 

Gene Name

 Slc3a2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
21	LNELEPEKQPMNAAD	ubiquitination	[1]
35	DGAAAGEKNGLVKIK	ubiquitination	[1]
42	KNGLVKIKVAEDEAE	ubiquitination	[1]
53	DEAEAGVKFTGLSKE	ubiquitination	[1]
59	VKFTGLSKEELLKVA	acetylation	[2]
59	VKFTGLSKEELLKVA	ubiquitination	[1]
148	LEYLSTLKVKGLVLG	acetylation	[2]
162	GPIHKNQKDEVNETD	acetylation	[2]
184	LGSQEDFKDLLQSAK	acetylation	[2]
191	KDLLQSAKKKSIHII	acetylation	[2]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. 

Sequence Annotation

 MOD_RES 2 2 Phosphoserine (By similarity).
 MOD_RES 58 58 Phosphoserine (By similarity).
 CARBOHYD 166 166 N-linked (GlcNAc...) (Potential).
 CARBOHYD 249 249 N-linked (GlcNAc...) (Potential).
 CARBOHYD 259 259 N-linked (GlcNAc...) (Potential).
 CARBOHYD 263 263 N-linked (GlcNAc...) (Potential).
 CARBOHYD 318 318 N-linked (GlcNAc...) (Potential).
 CARBOHYD 386 386 N-linked (GlcNAc...) (Potential).
 CARBOHYD 400 400 N-linked (GlcNAc...) (Potential).
 CARBOHYD 510 510 N-linked (GlcNAc...) (Potential).
 DISULFID 103 103 Interchain (with light chain) (By
 CROSSLNK 42 42 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Amino-acid transport; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 527 AA 

Protein Sequence

Gene Ontology

 GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IC:UniProtKB.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0043169; F:cation binding; IEA:InterPro.
 GO:0015175; F:neutral amino acid transmembrane transporter activity; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
 GO:0060356; P:leucine import; IDA:UniProtKB.
 GO:0015827; P:tryptophan transport; ISS:UniProtKB. 

Interpro

 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 

Pfam

 PF00128; Alpha-amylase 

SMART

  

PROSITE

  

PRINTS