CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001786
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Homoserine kinase 
Protein Synonyms/Alias
 HK; HSK 
Gene Name
 thrB 
Gene Synonyms/Alias
 b0003; JW0002 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
55NLGRFADKLPSEPREacetylation[1]
78RFCQELGKQIPVAMTacetylation[1]
224RQPELAAKLMKDVIAacetylation[1]
227ELAAKLMKDVIAEPYacetylation[1]
272TLFALCDKPETAQRVacetylation[1]
285RVADWLGKNYLQNQEacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate. Is also able to phosphorylate the hydroxy group on gamma-carbon of L-homoserine analogs when the functional group at the alpha-position is a carboxyl, an ester, or even a hydroxymethyl group. Neither L- threonine nor L-serine are substrates of the enzyme. 
Sequence Annotation
 NP_BIND 91 101 ATP (Potential).  
Keyword
 Amino-acid biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Reference proteome; Threonine biosynthesis; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 310 AA 
Protein Sequence
MVKVYAPASS ANMSVGFDVL GAAVTPVDGA LLGDVVTVEA AETFSLNNLG RFADKLPSEP 60
RENIVYQCWE RFCQELGKQI PVAMTLEKNM PIGSGLGSSA CSVVAALMAM NEHCGKPLND 120
TRLLALMGEL EGRISGSIHY DNVAPCFLGG MQLMIEENDI ISQQVPGFDE WLWVLAYPGI 180
KVSTAEARAI LPAQYRRQDC IAHGRHLAGF IHACYSRQPE LAAKLMKDVI AEPYRERLLP 240
GFRQARQAVA EIGAVASGIS GSGPTLFALC DKPETAQRVA DWLGKNYLQN QEGFVHICRL 300
DTAGARVLEN 310 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004413; F:homoserine kinase activity; IDA:EcoCyc.
 GO:0009088; P:threonine biosynthetic process; IMP:EcoCyc. 
Interpro
 IPR013750; GHMP_kinase_C_dom.
 IPR006204; GHMP_kinase_N_dom.
 IPR006203; GHMP_knse_ATP-bd_CS.
 IPR000870; Homoserine_kinase.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr. 
Pfam
 PF08544; GHMP_kinases_C
 PF00288; GHMP_kinases_N 
SMART
  
PROSITE
 PS00627; GHMP_KINASES_ATP 
PRINTS
 PR00958; HOMSERKINASE.