UniProt Accession

 PAPOA_BOVIN; P25500 

Genbank Protein ID

 X61585; X63436 

Genbank Nucleotide ID

 CAA43782.1; CAA45031.1 

Protein Name

 Poly(A) polymerase alpha 

Protein Synonyms/Alias

 PAP-alpha; Polynucleotide adenylyltransferase alpha 

Gene Name

 PAPOLA 

Gene Synonyms/Alias

 PAP 

Created Date

 July 27, 2013 

Organism

 Bos taurus (Bovine) 

NCBI Taxa ID

 9913 

Lysine Modification

Position	Peptide	Type	References
360	LSKAEWSKLFEAPNF	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus. 

Sequence Annotation

 NP_BIND 100 102 ATP.
 NP_BIND 113 115 ATP.
 NP_BIND 246 247 ATP.
 REGION 508 643 Ser/Thr-rich.
 REGION 671 739 Required for interaction with NUDT21.
 MOTIF 490 507 Nuclear localization signal 1.
 MOTIF 644 659 Nuclear localization signal 2.
 METAL 113 113 Magnesium 1; catalytic.
 METAL 113 113 Magnesium 2; catalytic.
 METAL 115 115 Magnesium 1; catalytic.
 METAL 115 115 Magnesium 2; catalytic.
 METAL 167 167 Magnesium 2; catalytic.
 BINDING 109 109 ATP.
 BINDING 167 167 ATP.
 BINDING 228 228 ATP.
 BINDING 237 237 ATP.
 MOD_RES 24 24 Phosphoserine (By similarity).
 MOD_RES 537 537 Phosphoserine; by MAPK (By similarity).
 MOD_RES 635 635 N6-acetyllysine.
 MOD_RES 644 644 N6-acetyllysine.
 MOD_RES 730 730 N6-acetyllysine; alternate.
 MOD_RES 734 734 N6-acetyllysine; alternate.
 CROSSLNK 444 444 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 445 445 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 506 506 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 507 507 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 730 730 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 734 734 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Isopeptide bond; Magnesium; Manganese; Metal-binding; mRNA processing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 739 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0030145; F:manganese ion binding; IDA:UniProtKB.
 GO:0004652; F:polynucleotide adenylyltransferase activity; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006378; P:mRNA polyadenylation; IBA:RefGenome.
 GO:0000398; P:mRNA splicing, via spliceosome; IBA:RefGenome.
 GO:0006369; P:termination of RNA polymerase II transcription; IBA:RefGenome. 

Interpro

 IPR002934; Nucleotidyltransferase.
 IPR011068; NuclTrfase_I_C.
 IPR007012; PolA_pol_cen_dom.
 IPR007010; PolA_pol_RNA-bd_dom.
 IPR014492; PolyA_polymerase. 

Pfam

 PF01909; NTP_transf_2
 PF04928; PAP_central
 PF04926; PAP_RNA-bind 

SMART

  

PROSITE

  

PRINTS