UniProt Accession

 LCAT_MOUSE; P16301; Q8K139 

Genbank Protein ID

 J05154; AK149476; AC159265; BC028861; X54095 

Genbank Nucleotide ID

 AAA39419.1; BAE28903.1; AAH28861.1; CAA38029.1 

Protein Name

 Phosphatidylcholine-sterol acyltransferase 

Protein Synonyms/Alias

 Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase 

Gene Name

 Lcat 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
129	IRVPGFGKTESVEYV	ubiquitination	[1]
262	IPILSNIKLKEEQRI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (By similarity). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. 

Sequence Annotation

 ACT_SITE 205 205 Charge relay system (By similarity).
 CARBOHYD 44 44 N-linked (GlcNAc...) (Potential).
 CARBOHYD 108 108 N-linked (GlcNAc...) (Potential).
 CARBOHYD 296 296 N-linked (GlcNAc...) (Potential).
 CARBOHYD 397 397 N-linked (GlcNAc...).
 CARBOHYD 408 408 N-linked (GlcNAc...).
 DISULFID 74 98 By similarity.
 DISULFID 337 380 By similarity.  

Keyword

 Acyltransferase; Cholesterol metabolism; Complete proteome; Disulfide bond; Glycoprotein; Lipid metabolism; Reference proteome; Secreted; Signal; Steroid metabolism; Sterol metabolism; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 438 AA 

Protein Sequence

Gene Ontology

 GO:0005615; C:extracellular space; IDA:MGI.
 GO:0034364; C:high-density lipoprotein particle; IEA:Compara.
 GO:0004607; F:phosphatidylcholine-sterol O-acyltransferase activity; IDA:MGI.
 GO:0034435; P:cholesterol esterification; IEA:Compara.
 GO:0042632; P:cholesterol homeostasis; IEA:Compara.
 GO:0008203; P:cholesterol metabolic process; IDA:MGI.
 GO:0030301; P:cholesterol transport; ISO:MGI.
 GO:0034375; P:high-density lipoprotein particle remodeling; IEA:Compara.
 GO:0042158; P:lipoprotein biosynthetic process; IDA:MGI.
 GO:0006656; P:phosphatidylcholine biosynthetic process; IEA:Compara.
 GO:0090107; P:regulation of high-density lipoprotein particle assembly; IDA:UniProtKB.
 GO:0043691; P:reverse cholesterol transport; IEA:Compara.
 GO:0034372; P:very-low-density lipoprotein particle remodeling; IEA:Compara. 

Interpro

 IPR003386; LACT/PDAT_acylTrfase. 

Pfam

 PF02450; LACT 

SMART

  

PROSITE

 PS00120; LIPASE_SER 

PRINTS