CPLM-010782

Genbank Nucleotide ID

Peptidyl-prolyl cis-trans isomerase FKBP3

Protein Synonyms/Alias

PPIase FKBP3; 25 kDa FK506-binding protein; 25 kDa FKBP; FKBP-25; FK506-binding protein 3; FKBP-3; Immunophilin FKBP25; Rapamycin-selective 25 kDa immunophilin; Rotamase

Homo sapiens (Human)

Position	Peptide	Type	References
27	LPKKDIIKFLQEHGS	ubiquitination	[1, 2]
42	DSFLAEHKLLGNIKN	ubiquitination	[1, 2, 3]
48	HKLLGNIKNVAKTAN	ubiquitination	[3]
80	KGTESISKVSEQVKN	ubiquitination	[4]
110	TLDEGPPKYTKSVLK	acetylation	[5]
160	AKKKKNAKPLSFKVG	acetylation	[4, 6]
165	NAKPLSFKVGVGKVI	ubiquitination	[3]
170	SFKVGVGKVIRGWDE	acetylation	[4, 5, 6, 7]
187	LTMSKGEKARLEIEP	methylation	[8]
200	EPEWAYGKKGQPDAK	acetylation	[4, 5, 6, 7]
201	PEWAYGKKGQPDAKI	ubiquitination	[9]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[8] Large-scale global identification of protein lysine methylation in vivo.
Cao XJ, Arnaudo AM, Garcia BA.
Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
[9] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.

DOMAIN 128 224 PPIase FKBP-type.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 36 36 Phosphoserine.
MOD_RES 152 152 Phosphoserine.
MOD_RES 170 170 N6-acetyllysine.

3D-structure; Acetylation; Complete proteome; Isomerase; Nucleus; Phosphoprotein; Reference proteome; Rotamase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016020; C:membrane; IBA:RefGenome.
GO:0005634; C:nucleus; IBA:RefGenome.
GO:0005528; F:FK506 binding; IBA:RefGenome.
GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IBA:RefGenome.
GO:0004872; F:receptor activity; TAS:ProtInc.
GO:0006457; P:protein folding; IEA:UniProtKB-KW.

IPR023566; PPIase_FKBP.
IPR001179; PPIase_FKBP_dom.

PS50059; FKBP_PPIASE