UniProt Accession

 NUCKS_HUMAN; Q9H1E3; Q54AC0; Q5PXE7; Q9H1D6; Q9H723 

Genbank Protein ID

 AJ012584; AB049824; AY823399; AJ237668; AK025133; CH471067; BC000805 

Genbank Nucleotide ID

 CAC20408.1; BAC06820.1; AAV83925.1; CAC20412.1; BAB15076.1; EAW91578.1; AAH00805.1 

Protein Name

 Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 

Protein Synonyms/Alias

 P1 

Gene Name

 NUCKS1 

Gene Synonyms/Alias

 NUCKS; JC7 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
9	SRPVRNRKVVDYSQF	acetylation	[1, 2]
9	SRPVRNRKVVDYSQF	methylation	[1]
35	RDSGPPTKKIRSSPR	acetylation	[2]
35	RDSGPPTKKIRSSPR	ubiquitination	[3]
52	KNKRRSGKNSQEDSE	acetylation	[1]
64	DSEDSEDKDVKTKKD	methylation	[1]
175	KMPKPRLKATVTPSP	acetylation	[1]
175	KMPKPRLKATVTPSP	methylation	[1]
175	KMPKPRLKATVTPSP	ubiquitination	[4]
184	TVTPSPVKGKGKVGR	acetylation	[1]
188	SPVKGKGKVGRPTAS	acetylation	[1, 4]
196	VGRPTASKASKEKTP	acetylation	[1]
218	EPESPPEKKTSTSPP	methylation	[1]
228	STSPPPEKSGDEGSE	phosphoglycerylation	[5]

Reference

 [1] Constitutive and dynamic phosphorylation and acetylation sites on NUCKS, a hypermodified nuclear protein, studied by quantitative proteomics.
 Wiśniewski JR, Zougman A, Krüger S, Ziółkowski P, Pudełko M, Bebenek M, Mann M.
 Proteins. 2008 Nov 15;73(3):710-8. [PMID: 18491381]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237] 

Functional Description

  

Sequence Annotation

 MOD_RES 14 14 Phosphoserine (By similarity).
 MOD_RES 19 19 Phosphoserine.
 MOD_RES 26 26 Phosphotyrosine.
 MOD_RES 54 54 Phosphoserine.
 MOD_RES 58 58 Phosphoserine.
 MOD_RES 61 61 Phosphoserine.
 MOD_RES 73 73 Phosphoserine.
 MOD_RES 75 75 Phosphoserine.
 MOD_RES 79 79 Phosphoserine.
 MOD_RES 113 113 Phosphoserine.
 MOD_RES 130 130 Phosphoserine.
 MOD_RES 132 132 Phosphoserine.
 MOD_RES 144 144 Phosphoserine.
 MOD_RES 179 179 Phosphothreonine.
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 202 202 Phosphothreonine.
 MOD_RES 204 204 Phosphoserine.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 223 223 Phosphoserine.
 MOD_RES 229 229 Phosphoserine.
 MOD_RES 234 234 Phosphoserine.
 MOD_RES 240 240 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 243 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. 

Interpro

 IPR026633; NUCKS1. 

Pfam

  

SMART

  

PROSITE

  

PRINTS