CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020385
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histone-lysine N-methyltransferase SETD2 
Protein Synonyms/Alias
 HIF-1; Huntingtin yeast partner B; Huntingtin-interacting protein 1; HIP-1; Huntingtin-interacting protein B; Lysine N-methyltransferase 3A; SET domain-containing protein 2; hSET2; p231HBP 
Gene Name
 SETD2 
Gene Synonyms/Alias
 HIF1; HYPB; KIAA1732; KMT3A; SET2; HSPC069 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42VQKTGFIKGPMFKGVubiquitination[1]
776DYSKTVVKEPVDTRVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. 
Sequence Annotation
 DOMAIN 1494 1548 AWS.
 DOMAIN 1550 1667 SET.
 DOMAIN 1674 1690 Post-SET.
 DOMAIN 2389 2422 WW.
 REGION 2137 2366 Low charge region.
 REGION 2457 2564 Interaction with POLR2A.
 MOD_RES 131 131 Phosphoserine.
 MOD_RES 321 321 Phosphoserine.
 MOD_RES 323 323 Phosphoserine.
 MOD_RES 624 624 Phosphoserine.
 MOD_RES 708 708 Phosphoserine.
 MOD_RES 744 744 Phosphoserine.
 MOD_RES 754 754 Phosphoserine.
 MOD_RES 1228 1228 Phosphoserine.
 MOD_RES 1872 1872 Phosphothreonine.
 MOD_RES 2080 2080 Phosphoserine.
 MOD_RES 2082 2082 Phosphoserine.  
Keyword
 3D-structure; Activator; Alternative splicing; Chromatin regulator; Chromosome; Coiled coil; Complete proteome; DNA-binding; Methyltransferase; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transcription; Transcription regulation; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2564 AA 
Protein Sequence
MKQLQPQPPP KMGDFYDPEH PTPEEEENEA KIENVQKTGF IKGPMFKGVA SSRFLPKGTK 60
TKVNLEEQGR QKVSFSFSLT KKTLQNRFLT ALGNEKQSDT PNPPAVPLQV DSTPKMKMEI 120
GDTLSTAEES SPPKSRVELG KIHFKKHLLH VTSRPLLATT TAVASPPTHA APLPAVIAES 180
TTVDSPPSSP PPPPPPAQAT TLSSPAPVTE PVALPHTPIT VLMAAPVPLP VDVAVRSLKE 240
PPIIIVPESL EADTKQDTIS NSLEEHVTQI LNEQADISSK KEDSHIGKDE EIPDSSKISL 300
SCKKTGSKKK SSQSEGIFLG SESDEDSVRT SSSQRSHDLK FSASIEKERD FKKSSAPLKS 360
EDLGKPSRSK TDRDDKYFSY SKLERDTRYV SSRCRSERER RRSRSHSRSE RGSRTNLSYS 420
RSERSHYYDS DRRYHRSSPY RERTRYSRPY TDNRARESSD SEEEYKKTYS RRTSSHSSSY 480
RDLRTSSYSK SDRDCKTETS YLEMERRGKY SSKLERESKR TSENEAIKRC CSPPNELGFR 540
RGSSYSKHDS SASRYKSTLS KPIPKSDKFK NSFCCTELNE EIKQSHSFSL QTPCSKGSEL 600
RMINKNPERE KAGSPAPSNR LNDSPTLKKL DELPIFKSEF ITHDSHDSIK ELDSLSKVKN 660
DQLRSFCPIE LNINGSPGAE SDLATFCTSK TDAVLMTSDD SVTGSELSPL VKACMLSSNG 720
FQNISRCKEK DLDDTCMLHK KSESPFRETE PLVSPHQDKL MSMPVMTVDY SKTVVKEPVD 780
TRVSCCKTKD SDIYCTLNDS NPSLCNSEAE NIEPSVMKIS SNSFMNVHLE SKPVICDSRN 840
LTDHSKFACE EYKQSIGSTS SASVNHFDDL YQPIGSSGIA SSLQSLPPGI KVDSLTLLKC 900
GENTSPVLDA VLKSKKSSEF LKHAGKETIV EVGSDLPDSG KGFASRENRR NNGLSGKCLQ 960
EAQEEGNSIL PERRGRPEIS LDERGEGGHV HTSDDSEVVF SSCDLNLTME DSDGVTYALK 1020
CDSSGHAPEI VSTVHEDYSG SSESSNDESD SEDTDSDDSS IPRNRLQSVV VVPKNSTLPM 1080
EETSPCSSRS SQSYRHYSDH WEDERLESRR HLYEEKFESI ASKACPQTDK FFLHKGTEKN 1140
PEISFTQSSR KQIDNRLPEL SHPQSDGVDS TSHTDVKSDP LGHPNSEETV KAKIPSRQQE 1200
ELPIYSSDFE DVPNKSWQQT TFQNRPDSRL GKTELSFSSS CEIPHVDGLH SSEELRNLGW 1260
DFSQEKPSTT YQQPDSSYGA CGGHKYQQNA EQYGGTRDYW QGNGYWDPRS GRPPGTGVVY 1320
DRTQGQVPDS LTDDREEEEN WDQQDGSHFS DQSDKFLLSL QKDKGSVQAP EISSNSIKDT 1380
LAVNEKKDFS KNLEKNDIKD RGPLKKRRQE IESDSESDGE LQDRKKVRVE VEQGETSVPP 1440
GSALVGPSCV MDDFRDPQRW KECAKQGKMP CYFDLIEENV YLTERKKNKS HRDIKRMQCE 1500
CTPLSKDERA QGEIACGEDC LNRLLMIECS SRCPNGDYCS NRRFQRKQHA DVEVILTEKK 1560
GWGLRAAKDL PSNTFVLEYC GEVLDHKEFK ARVKEYARNK NIHYYFMALK NDEIIDATQK 1620
GNCSRFMNHS CEPNCETQKW TVNGQLRVGF FTTKLVPSGS ELTFDYQFQR YGKEAQKCFC 1680
GSANCRGYLG GENRVSIRAA GGKMKKERSR KKDSVDGELE ALMENGEGLS DKNQVLSLSR 1740
LMVRIETLEQ KLTCLELIQN THSQSCLKSF LERHGLSLLW IWMAELGDGR ESNQKLQEEI 1800
IKTLEHLPIP TKNMLEESKV LPIIQRWSQT KTAVPPLSEG DGYSSENTSR AHTPLNTPDP 1860
STKLSTEADT DTPKKLMFRR LKIISENSMD SAISDATSEL EGKDGKEDLD QLENVPVEEE 1920
EELQSQQLLP QQLPECKVDS ETNIEASKLP TSEPEADAEI EPKESNGTKL EEPINEETPS 1980
QDEEEGVSDV ESERSQEQPD KTVDISDLAT KLLDSWKDLK EVYRIPKKSQ TEKENTTTER 2040
GRDAVGFRDQ TPAPKTPNRS RERDPDKQTQ NKEKRKRRSS LSPPSSAYER GTKRPDDRYD 2100
TPTSKKKVRI KDRNKLSTEE RRKLFEQEVA QREAQKQQQQ MQNLGMTSPL PYDSLGYNAP 2160
HHPFAGYPPG YPMQAYVDPS NPNAGKVLLP TPSMDPVCSP APYDHAQPLV GHSTEPLSAP 2220
PPVPVVPHVA APVEVSSSQY VAQSDGVVHQ DSSVAVLPVP APGPVQGQNY SVWDSNQQSV 2280
SVQQQYSPAQ SQATIYYQGQ TCPTVYGVTS PYSQTTPPIV QSYAQPSLQY IQGQQIFTAH 2340
PQGVVVQPAA AVTTIVAPGQ PQPLQPSEMV VTNNLLDLPP PSPPKPKTIV LPPNWKTARD 2400
PEGKIYYYHV ITRQTQWDPP TWESPGDDAS LEHEAEMDLG TPTYDENPMK ASKKPKTAEA 2460
DTSSELAKKS KEVFRKEMSQ FIVQCLNPYR KPDCKVGRIT TTEDFKHLAR KLTHGVMNKE 2520
LKYCKNPEDL ECNENVKHKT KEYIKKYMQK FGAVYKPKED TELE 2564 
Gene Ontology
 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0018024; F:histone-lysine N-methyltransferase activity; IEA:EC.
 GO:0016491; F:oxidoreductase activity; IEA:InterPro.
 GO:0046914; F:transition metal ion binding; IEA:InterPro.
 GO:0001525; P:angiogenesis; IEA:Compara.
 GO:0035441; P:cell migration involved in vasculogenesis; IEA:Compara.
 GO:0060977; P:coronary vasculature morphogenesis; IEA:Compara.
 GO:0048701; P:embryonic cranial skeleton morphogenesis; IEA:Compara.
 GO:0060669; P:embryonic placenta morphogenesis; IEA:Compara.
 GO:0030900; P:forebrain development; IEA:Compara.
 GO:0010452; P:histone H3-K36 methylation; IEA:Compara.
 GO:0048332; P:mesoderm morphogenesis; IEA:Compara.
 GO:0001763; P:morphogenesis of a branching structure; IEA:Compara.
 GO:0001843; P:neural tube closure; IEA:Compara.
 GO:0018023; P:peptidyl-lysine trimethylation; IEA:Compara.
 GO:0060039; P:pericardium development; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0048864; P:stem cell development; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR006560; AWS.
 IPR009078; Ferritin-like_SF.
 IPR003616; Post-SET_dom.
 IPR001214; SET_dom.
 IPR013257; SRI.
 IPR001202; WW_dom. 
Pfam
 PF00856; SET
 PF08236; SRI
 PF00397; WW 
SMART
 SM00570; AWS
 SM00508; PostSET
 SM00317; SET
 SM00456; WW 
PROSITE
 PS51215; AWS
 PS50868; POST_SET
 PS50280; SET
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS