UniProt Accession

 YDFG_ECOLI; P39831; P77149; P78161; P78162 

Genbank Protein ID

 U00096; AP009048; X57947 

Genbank Nucleotide ID

 AAC74612.1; BAA15241.1 

Protein Name

 NADP-dependent 3-hydroxy acid dehydrogenase YdfG 

Protein Synonyms/Alias

 Malonic semialdehyde reductase 

Gene Name

 ydfG 

Gene Synonyms/Alias

 b1539; JW1532 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
26	RFIQQGHKVIATGRR	acetylation	[1, 2]
41	QERLQELKDELGDNL	acetylation	[2]
151	GNVYGATKAFVRQFS	acetylation	[2]
191	EFSNVRFKGDDGKAE	acetylation	[2]
196	RFKGDDGKAEKTYQN	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Has a broad substrate specificity and is able to catalyze the reduction of the malonic semialdehyde to 3- hydroxypropionic acid. YdfG is apparently supplementing RutE, the presumed malonic semialdehyde reductase involved in pyrimidine degradation since both are able to detoxify malonic semialdehyde. Also catalyzes the NADP-dependent oxidation of L-allo-threonine to L-2-amino-3-keto-butyrate, which is spontaneously decarboxylated into aminoacetone. Also acts on D-threonine, L-serine, D-serine, D-3-hydroxyisobutyrate, L-3-hydroxyisobutyrate, D-glycerate and L- glycerate. Although usually assayed in vitro as an oxidase, YdfG is presumed to be a reductase in vivo. 

Sequence Annotation

 NP_BIND 4 28 NADP (By similarity).
 ACT_SITE 147 147 Proton acceptor (By similarity).
 BINDING 134 134 Substrate (By similarity).  

Keyword

 Complete proteome; Direct protein sequencing; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 248 AA 

Protein Sequence

Gene Ontology

 GO:0035527; F:3-hydroxypropionate dehydrogenase (NADP+) activity; IDA:EcoCyc.
 GO:0031132; F:serine 3-dehydrogenase activity; IDA:EcoCyc.
 GO:0006212; P:uracil catabolic process; IDA:UniProtKB. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

 PF00106; adh_short 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.