UniProt Accession

 OBG_ECOLI; P42641; Q2M929 

Genbank Protein ID

 U18997; U00096; AP009048 

Genbank Nucleotide ID

 AAA57984.1; AAC76215.1; BAE77227.1 

Protein Name

 GTPase ObgE/CgtA 

Protein Synonyms/Alias

 GTP-binding protein Obg 

Gene Name

 obgE 

Gene Synonyms/Alias

 cgtA; obg; yhbZ; b3183; JW3150 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
110	ETMGDMTKHGQRLLV	acetylation	[1]
268	IIISELEKYSQDLAT	acetylation	[1]
290	NKIDLLDKVEAEEKA	acetylation	[1]
348	EEAKQPEKVEFMWDD	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 An abundant, essential GTPase which binds GTP, GDP and ppGpp with moderate affinity, shows high guanine nucleotide exchange rate constants for GTP and GDP, and has a relatively low GTP hydrolysis rate. It is estimated there are 34000 molecules in log-phase cells and 5600 molecules in stationary-phase cells as the monomer. Required for chromosome segregation. It also plays a role in the stringent response, and a non-essential role in the late steps of ribosome biogenesis, but how it performs these functions remains unelucidated. 

Sequence Annotation

 DOMAIN 171 284 G.
 NP_BIND 166 173 GTP (Probable).
 NP_BIND 213 217 GTP (By similarity).
 NP_BIND 283 286 GTP (By similarity).  

Keyword

 Complete proteome; Cytoplasm; DNA-binding; GTP-binding; Nucleotide-binding; Reference proteome; RNA-binding; rRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 390 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:EcoCyc.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0019003; F:GDP binding; IDA:EcoCyc.
 GO:0005525; F:GTP binding; IDA:EcoCyc.
 GO:0003924; F:GTPase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IMP:EcoCyc.
 GO:0042255; P:ribosome assembly; IMP:EcoCyc. 

Interpro

 IPR014100; GTP-bd_Obg/CgtA.
 IPR006074; GTP1-OBG_CS.
 IPR006169; GTP1_OBG_dom.
 IPR006073; GTP_binding_domain.
 IPR027417; P-loop_NTPase. 

Pfam

 PF01018; GTP1_OBG
 PF01926; MMR_HSR1 

SMART

  

PROSITE

 PS00905; GTP1_OBG 

PRINTS

 PR00326; GTP1OBG.