| Tag | Content |
|---|
| CPLM ID | CPLM-018977 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | DNA topoisomerase I, mitochondrial |
Protein Synonyms/Alias | TOP1mt |
Gene Name | TOP1MT |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 320 | VALYFIDKLALRAGN | ubiquitination | [1] | | 329 | ALRAGNEKEDGEAAD | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). |
Sequence Annotation | REGION 261 262 Interaction with DNA (By similarity).
REGION 324 329 Interaction with DNA (By similarity).
REGION 421 423 Interaction with DNA (By similarity).
ACT_SITE 559 559 O-(3'-phospho-DNA)-tyrosine intermediate |
Keyword | Alternative splicing; ATP-binding; Complete proteome; DNA-binding; Isomerase; Mitochondrion; Nucleotide-binding; Polymorphism; Reference proteome; Topoisomerase; Transit peptide. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 601 AA |
Protein Sequence | MRVVRLLRLR AALTLLGEVP RRPASRGVPG SRRTQKGSGA RWEKEKHEDG VKWRQLEHKG 60
PYFAPPYEPL PDGVRFFYEG RPVRLSVAAE EVATFYGRML DHEYTTKEVF RKNFFNDWRK 120
EMAVEEREVI KSLDKCDFTE IHRYFVDKAA ARKVLSREEK QKLKEEAEKL QQEFGYCILD 180
GHQEKIGNFK IEPPGLFRGR GDHPKMGMLK RRITPEDVVI NCSRDSKIPE PPAGHQWKEV 240
RSDNTVTWLA AWTESVQNSI KYIMLNPCSK LKGETAWQKF ETARRLRGFV DEIRSQYRAD 300
WKSREMKTRQ RAVALYFIDK LALRAGNEKE DGEAADTVGC CSLRVEHVQL HPEADGCQHV 360
VEFDFLGKDC IRYYNRVPVE KPVYKNLQLF MENKDPRDDL FDRLTTTSLN KHLQELMDGL 420
TAKVFRTYNA SITLQEQLRA LTRAEDSIAA KILSYNRANR VVAILCNHQR ATPSTFEKSM 480
QNLQTKIQAK KEQVAEARAE LRRARAEHKA QGDGKSRSVL EKKRRLLEKL QEQLAQLSVQ 540
ATDKEENKQV ALGTSKLNYL DPRISIAWCK RFRVPVEKIY SKTQRERFAW ALAMAGEDFE 600
F 601 |
Gene Ontology | GO:0005694; C:chromosome; IEA:InterPro. GO:0042645; C:mitochondrial nucleoid; IDA:BHF-UCL. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0003917; F:DNA topoisomerase type I activity; IBA:RefGenome. GO:0003918; F:DNA topoisomerase type II (ATP-hydrolyzing) activity; IEA:InterPro. GO:0006260; P:DNA replication; IBA:RefGenome. GO:0006265; P:DNA topological change; IBA:RefGenome. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |