CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015369
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 POTE ankyrin domain family member E 
Protein Synonyms/Alias
 ANKRD26-like family C member 1A; Prostate, ovary, testis-expressed protein on chromosome 2; POTE-2 
Gene Name
 POTEE 
Gene Synonyms/Alias
 A26C1A; POTE2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15MPAASSVKKPFGLRSubiquitination[1]
16PAASSVKKPFGLRSKubiquitination[1]
55DHDDSAMKTLRSKMGubiquitination[1, 2, 3]
77PCCRGSGKSNVGASGubiquitination[1]
92DHDDSAMKTLRNKMGubiquitination[1, 2, 3]
114PCCRGSGKSKVGAWGubiquitination[2, 4]
116CRGSGKSKVGAWGDYubiquitination[1, 2, 3, 4]
142VRGEDLDKLHRAAWWubiquitination[1, 2]
270ADIESKNKHGLTPLLubiquitination[1]
295VKFLIKKKANLNALDubiquitination[5]
684LEDIESVKKKNDNLLubiquitination[4, 6]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265
Functional Description
  
Sequence Annotation
 REPEAT 172 201 ANK 1.
 REPEAT 205 234 ANK 2.
 REPEAT 238 267 ANK 3.
 REPEAT 271 300 ANK 4.
 REPEAT 304 333 ANK 5.
 REGION 702 1075 Actin-like.
 CROSSLNK 684 684 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; ANK repeat; Coiled coil; Complete proteome; Isopeptide bond; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1075 AA 
Protein Sequence
MVVEVDSMPA ASSVKKPFGL RSKMGKWCCR CFPCYRESGK SNVGTSGDHD DSAMKTLRSK 60
MGKWCHHCFP CCRGSGKSNV GASGDHDDSA MKTLRNKMGK WCCHCFPCCR GSGKSKVGAW 120
GDYDDSAFME PRYHVRGEDL DKLHRAAWWG KVPRKDLIVM LRDTDVNKKD KQKRTALHLA 180
SANGNSEVVK LLLDRRCQLN VLDNKKRTAL IKAVQCQEDE CALMLLEHGT DPNIPDEYGN 240
TTLHYAIYNE DKLMAKALLL YGADIESKNK HGLTPLLLGV HEQKQQVVKF LIKKKANLNA 300
LDRYGRTALI LAVCCGSASI VSLLLEQNID VSSQDLSGQT AREYAVSSHH HVICQLLSDY 360
KEKQMLKISS ENSNPEQELK LTSEEESQRF KGSENSQPEK MSQELEINKD GDREVEEEMK 420
KHESNNVGLL ENLTNGVTAG NGDNGLIPQR KSRTPENQQF PDNESEEYHR ICELLSDYKE 480
KQMPKYSSEN SNPEQDLKLT SEEESQRLKG SENGQPEKRS QEPEINKDGD RELENFMAIE 540
EMKKHGSTHV GFPENLTNGA TAGNGDDGLI PPRKSRTPES QQFPDTENEE YHSDEQNDTQ 600
KQFCEEQNTG ILHDEILIHE EKQIEVVEKM NSELSLSCKK EKDVLHENST LREEIAMLRL 660
ELDTMKHQSQ LREKKYLEDI ESVKKKNDNL LKALQLNELT MDDDTAVLVI DNGSGMCKAG 720
FAGDDAPRAV FPSIVGRPRQ QGMMGGMHQK ESYVGKEAQS KRGILTLKYP MEHGIITNWD 780
DMEKIWHHTF YNELRVAPEE HPILLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVP 840
SLYTSGRTTG IVMDSGDGVT HTVPIYEGNA LPHATLRLDL AGRELPDYLM KILTERGYRF 900
TTMAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA 960
LFQPCFLGME SCGIHETTFN SIMKSDVDIR KDLYTNTVLS GGTTMYPGMA HRMQKEIAAL 1020
APSMMKIRII APPKRKYSVW VGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF 1075 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro. 
Interpro
 IPR004000; Actin-related.
 IPR020902; Actin/actin-like_CS.
 IPR004001; Actin_CS.
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom. 
Pfam
 PF00022; Actin
 PF00023; Ank
 PF12796; Ank_2 
SMART
 SM00268; ACTIN
 SM00248; ANK 
PROSITE
 PS00432; ACTINS_2
 PS01132; ACTINS_ACT_LIKE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS
 PR00190; ACTIN.