UniProt Accession

 EED_HUMAN; O75530; A8K7V5; O00149; Q6NTH2; Q7LDA5; Q7LDG8; Q86VV2; Q9UNY7 

Genbank Protein ID

 AF080227; AF070418; U90651; AF099032; AK292120; AP003084; CH471076; CH471076; BC047672; BC068995; AF078933 

Genbank Nucleotide ID

 AAC95144.1; AAC23685.1; AAD08714.1; AAD08815.1; BAF84809.1; EAW75129.1; EAW75130.1; AAH47672.1; AAH68995.1; AAC68675.1 

Protein Name

 Polycomb protein EED 

Protein Synonyms/Alias

 hEED; WD protein associating with integrin cytoplasmic tails 1; WAIT-1 

Gene Name

 EED 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	GTDMPAAKKQKLSSD	ubiquitination	[1]
211	NLLLSVSKDHALRLW	ubiquitination	[1]
275	KRMMNAIKESYDYNP	ubiquitination	[2]
284	SYDYNPNKTNRPFIS	ubiquitination	[2, 3, 4]
344	EDDIDKIKPSESNVT	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. 

Sequence Annotation

 REPEAT 91 134 WD 1.
 REPEAT 142 185 WD 2.
 REPEAT 188 228 WD 3.
 REPEAT 234 275 WD 4.
 REPEAT 304 341 WD 5.
 REPEAT 359 399 WD 6.
 REPEAT 408 441 WD 7.
 REGION 81 441 Interaction with EZH2 (By similarity).
 REGION 149 303 Required for interaction with the matrix
 REGION 301 441 Required for interaction with the matrix
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 55 55 Phosphothreonine.
 MOD_RES 66 66 N6,N6,N6-trimethyllysine; alternate.
 MOD_RES 66 66 N6,N6-dimethyllysine; alternate.
 MOD_RES 66 66 N6-methyllysine; alternate.
 MOD_RES 197 197 N6,N6,N6-trimethyllysine; alternate.
 MOD_RES 197 197 N6,N6-dimethyllysine; alternate.
 MOD_RES 197 197 N6-methyllysine; alternate.
 MOD_RES 268 268 N6,N6,N6-trimethyllysine; alternate.
 MOD_RES 268 268 N6,N6-dimethyllysine; alternate.
 MOD_RES 268 268 N6-methyllysine; alternate.
 MOD_RES 284 284 N6,N6,N6-trimethyllysine; alternate.
 MOD_RES 284 284 N6,N6-dimethyllysine; alternate.
 MOD_RES 284 284 N6-methyllysine; alternate.  

Keyword

 3D-structure; Acetylation; Alternative initiation; Alternative splicing; Chromatin regulator; Chromosome; Complete proteome; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 441 AA 

Protein Sequence

Gene Ontology

 GO:0035098; C:ESC/E(Z) complex; IDA:UniProtKB.
 GO:0045120; C:pronucleus; IEA:Compara.
 GO:0001739; C:sex chromatin; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0042054; F:histone methyltransferase activity; IDA:MGI.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0006349; P:regulation of gene expression by genetic imprinting; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS