CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005398
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tumor necrosis factor receptor superfamily member 6 
Protein Synonyms/Alias
 Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD95 
Gene Name
 FAS 
Gene Synonyms/Alias
 APT1; FAS1; TNFRSF6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
263EAKIDEIKNDNVQDTubiquitination[1, 2]
288WHQLHGKKEAYDTLIubiquitination[1]
309NLCTLAEKIQTIILKubiquitination[3]
316KIQTIILKDITSDSEubiquitination[1, 2, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). 
Sequence Annotation
 REPEAT 47 83 TNFR-Cys 1.
 REPEAT 84 127 TNFR-Cys 2.
 REPEAT 128 166 TNFR-Cys 3.
 DOMAIN 230 314 Death.
 REGION 212 317 Interaction with HIPK3 (By similarity).
 MOD_RES 209 209 Phosphoserine.
 MOD_RES 214 214 Phosphothreonine (By similarity).
 MOD_RES 225 225 Phosphoserine (By similarity).
 MOD_RES 230 230 Phosphoserine (By similarity).
 CARBOHYD 28 28 O-linked (GalNAc...).
 CARBOHYD 118 118 N-linked (GlcNAc...).
 CARBOHYD 136 136 N-linked (GlcNAc...) (Potential).
 DISULFID 59 73 By similarity.
 DISULFID 63 82 By similarity.
 DISULFID 85 101 By similarity.
 DISULFID 104 119 By similarity.
 DISULFID 107 127 By similarity.
 DISULFID 129 143 By similarity.
 DISULFID 146 157 By similarity.
 DISULFID 149 165 By similarity.  
Keyword
 3D-structure; Alternative splicing; Apoptosis; Cell membrane; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Secreted; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 335 AA 
Protein Sequence
MLGIWTLLPL VLTSVARLSS KSVNAQVTDI NSKGLELRKT VTTVETQNLE GLHHDGQFCH 60
KPCPPGERKA RDCTVNGDEP DCVPCQEGKE YTDKAHFSSK CRRCRLCDEG HGLEVEINCT 120
RTQNTKCRCK PNFFCNSTVC EHCDPCTKCE HGIIKECTLT SNTKCKEEGS RSNLGWLCLL 180
LLPIPLIVWV KRKEVQKTCR KHRKENQGSH ESPTLNPETV AINLSDVDLS KYITTIAGVM 240
TLSQVKGFVR KNGVNEAKID EIKNDNVQDT AEQKVQLLRN WHQLHGKKEA YDTLIKDLKK 300
ANLCTLAEKI QTIILKDITS DSENSNFRNE IQSLV 335 
Gene Ontology
 GO:0031265; C:CD95 death-inducing signaling complex; IDA:UniProtKB.
 GO:0005829; C:cytosol; NAS:UniProtKB.
 GO:0009897; C:external side of plasma membrane; IEA:Compara.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0045121; C:membrane raft; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0004872; F:receptor activity; NAS:UniProtKB.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; TAS:Reactome.
 GO:0006924; P:activation-induced cell death of T cells; IEA:Compara.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0019724; P:B cell mediated immunity; IEA:Compara.
 GO:0071455; P:cellular response to hyperoxia; IMP:UniProtKB.
 GO:0071285; P:cellular response to lithium ion; IEA:Compara.
 GO:0071260; P:cellular response to mechanical stimulus; IEP:UniProtKB.
 GO:0008625; P:extrinsic apoptotic signaling pathway via death domain receptors; IEA:Compara.
 GO:0010467; P:gene expression; IEA:Compara.
 GO:0002377; P:immunoglobulin production; IEA:Compara.
 GO:0006917; P:induction of apoptosis; TAS:ProtInc.
 GO:0006925; P:inflammatory cell apoptotic process; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; TAS:ProtInc.
 GO:0050869; P:negative regulation of B cell activation; IEA:Compara.
 GO:0045060; P:negative thymic T cell selection; IEA:Compara.
 GO:0051402; P:neuron apoptotic process; IEA:Compara.
 GO:0010940; P:positive regulation of necrotic cell death; IMP:BHF-UCL.
 GO:0032464; P:positive regulation of protein homooligomerization; IEA:Compara.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0045619; P:regulation of lymphocyte differentiation; IEA:Compara.
 GO:0045637; P:regulation of myeloid cell differentiation; IEA:Compara.
 GO:0003014; P:renal system process; IEA:Compara.
 GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara.
 GO:0009636; P:response to toxic substance; IEA:Compara.
 GO:0048536; P:spleen development; IEA:Compara.
 GO:0006927; P:transformed cell apoptotic process; IEA:Compara. 
Interpro
 IPR011029; DEATH-like_dom.
 IPR000488; Death_domain.
 IPR008063; Fas_rcpt.
 IPR001368; TNFR/NGFR_Cys_rich_reg. 
Pfam
 PF00531; Death
 PF00020; TNFR_c6 
SMART
 SM00005; DEATH
 SM00208; TNFR 
PROSITE
 PS50017; DEATH_DOMAIN
 PS00652; TNFR_NGFR_1
 PS50050; TNFR_NGFR_2 
PRINTS
 PR01680; TNFACTORR6.