CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001817
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoenolpyruvate carboxylase 
Protein Synonyms/Alias
 PEPC; PEPCase 
Gene Name
 ppc 
Gene Synonyms/Alias
 glu; b3956; JW3928 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
17SNVSMLGKVLGETIKacetylation[1, 2]
24KVLGETIKDALGEHIacetylation[1, 2]
94QYHSISPKGEAASNPacetylation[2]
112ARTLRKLKNQPELSEacetylation[2]
123ELSEDTIKKAVESLSacetylation[2]
164CLKQLDNKDIADYEHacetylation[2]
193WHTDEIRKLRPSPVDacetylation[1]
203PSPVDEAKWGFAVVEacetylation[2]
235LEENLGYKLPVEFVPacetylation[2]
274VLLLSRWKATDLFLKacetylation[2]
317EPYRYLMKNLRSRLMacetylation[1, 2]
384LDTLRRVKCFGVPLVacetylation[2]
437LIRELNSKRPLLPRNacetylation[2]
570ALIKTCEKAGIELTLacetylation[2]
605SQPPGSLKGGLRVTEacetylation[2]
676RGYVRENKDFVPYFRacetylation[2]
693TPEQELGKLPLGSRPacetylation[2]
740GAGTALQKVVEDGKQacetylation[2]
746QKVVEDGKQSELEAMacetylation[2]
789YDQRLVDKALWPLGKacetylation[1, 2]
796KALWPLGKELRNLQEacetylation[2]
855HRSRQAEKEGQEPDPacetylation[2]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. 
Sequence Annotation
 ACT_SITE 138 138
 ACT_SITE 546 546 By similarity.  
Keyword
 3D-structure; Allosteric enzyme; Carbon dioxide fixation; Complete proteome; Lyase; Magnesium; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 883 AA 
Protein Sequence
MNEQYSALRS NVSMLGKVLG ETIKDALGEH ILERVETIRK LSKSSRAGND ANRQELLTTL 60
QNLSNDELLP VARAFSQFLN LANTAEQYHS ISPKGEAASN PEVIARTLRK LKNQPELSED 120
TIKKAVESLS LELVLTAHPT EITRRTLIHK MVEVNACLKQ LDNKDIADYE HNQLMRRLRQ 180
LIAQSWHTDE IRKLRPSPVD EAKWGFAVVE NSLWQGVPNY LRELNEQLEE NLGYKLPVEF 240
VPVRFTSWMG GDRDGNPNVT ADITRHVLLL SRWKATDLFL KDIQVLVSEL SMVEATPELL 300
ALVGEEGAAE PYRYLMKNLR SRLMATQAWL EARLKGEELP KPEGLLTQNE ELWEPLYACY 360
QSLQACGMGI IANGDLLDTL RRVKCFGVPL VRIDIRQEST RHTEALGELT RYLGIGDYES 420
WSEADKQAFL IRELNSKRPL LPRNWQPSAE TREVLDTCQV IAEAPQGSIA AYVISMAKTP 480
SDVLAVHLLL KEAGIGFAMP VAPLFETLDD LNNANDVMTQ LLNIDWYRGL IQGKQMVMIG 540
YSDSAKDAGV MAASWAQYQA QDALIKTCEK AGIELTLFHG RGGSIGRGGA PAHAALLSQP 600
PGSLKGGLRV TEQGEMIRFK YGLPEITVSS LSLYTGAILE ANLLPPPEPK ESWRRIMDEL 660
SVISCDVYRG YVRENKDFVP YFRSATPEQE LGKLPLGSRP AKRRPTGGVE SLRAIPWIFA 720
WTQNRLMLPA WLGAGTALQK VVEDGKQSEL EAMCRDWPFF STRLGMLEMV FAKADLWLAE 780
YYDQRLVDKA LWPLGKELRN LQEEDIKVVL AIANDSHLMA DLPWIAESIQ LRNIYTDPLN 840
VLQAELLHRS RQAEKEGQEP DPRVEQALMV TIAGIAAGMR NTG 883 
Gene Ontology
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:HAMAP.
 GO:0015977; P:carbon fixation; IEA:HAMAP.
 GO:0006107; P:oxaloacetate metabolic process; IEA:HAMAP.
 GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro. 
Interpro
 IPR021135; PEP_COase.
 IPR018129; PEP_COase_AS.
 IPR022805; PEP_COase_bac/pln-type.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom. 
Pfam
 PF00311; PEPcase 
SMART
  
PROSITE
 PS00781; PEPCASE_1
 PS00393; PEPCASE_2 
PRINTS
 PR00150; PEPCARBXLASE.