CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000653
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TELO2-interacting protein 1 homolog 
Protein Synonyms/Alias
 Protein SMG10 
Gene Name
 TTI1 
Gene Synonyms/Alias
 KIAA0406; SMG10 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
257EQLKRISKVQAKPAVubiquitination[1]
261RISKVQAKPAVEHRVubiquitination[2]
353EIQAQCNKVLRHFADubiquitination[1, 3]
362LRHFADQKVVVGNKAubiquitination[1]
368QKVVVGNKALADILSubiquitination[1]
461DDLNASPKTSATQPWubiquitination[1, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals. 
Sequence Annotation
 MOD_RES 459 459 Phosphoserine.
 MOD_RES 828 828 Phosphoserine; by CK2.  
Keyword
 Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1089 AA 
Protein Sequence
MAVFDTPEEA FGVLRPVCVQ LTKTQTVENV EHLQTRLQAV SDSALQELQQ YILFPLRFTL 60
KTPGPKRERL IQSVVECLTF VLSSTCVKEQ ELLQELFSEL SACLYSPSSQ KPAAVSEELK 120
LAVIQGLSTL MHSAYGDIIL TFYEPSILPR LGFAVSLLLG LAEQEKSKQI KIAALKCLQV 180
LLLQCDCQDH PRSLDELEQK QLGDLFASFL PGISTALTRL ITGDFKQGHS IVVSSLKIFY 240
KTVSFIMADE QLKRISKVQA KPAVEHRVAE LMVYREADWV KKTGDKLTIL IKKIIECVSV 300
HPHWKVRLEL VELVEDLLLK CSQSLVECAG PLLKALVGLV NDESPEIQAQ CNKVLRHFAD 360
QKVVVGNKAL ADILSESLHS LATSLPRLMN SQDDQGKFST LSLLLGYLKL LGPKINFVLN 420
SVAHLQRLSK ALIQVLELDV ADIKIVEERR WNSDDLNASP KTSATQPWNR IQRRYFRFFT 480
DERIFMLLRQ VCQLLGYYGN LYLLVDHFME LYHQSVVYRK QAAMILNELV TGAAGLEVED 540
LHEKHIKTNP EELREIVTSI LEEYTSQENW YLVTCLETEE MGEELMMEHP GLQAITSGEH 600
TCQVTSFLAF SKPSPTICSM NSNIWQICIQ LEGIGQFAYA LGKDFCLLLM SALYPVLEKA 660
GDQTLLISQV ATSTMMDVCR ACGYDSLQHL INQNSDYLVN GISLNLRHLA LHPHTPKVLE 720
VMLRNSDANL LPLVADVVQD VLATLDQFYD KRAASFVSVL HALMAALAQW FPDTGNLGHL 780
QEQSLGEEGS HLNQRPAALE KSTTTAEDIE QFLLNYLKEK DVADGNVSDF DNEEEEQSVP 840
PKVDENDTRP DVEPPLPLQI QIAMDVMERC IHLLSDKNLQ IRLKVLDVLD LCVVVLQSHK 900
NQLLPLAHQA WPSLVHRLTR DAPLAVLRAF KVLRTLGSKC GDFLRSRFCK DVLPKLAGSL 960
VTQAPISARA GPVYSHTLAF KLQLAVLQGL GPLCERLDLG EGDLNKVADA CLIYLSVKQP 1020
VKLQEAARSV FLHLMKVDPD STWFLLNELY CPVQFTPPHP SLHPVQLHGA SGQQNPYTTN 1080
VLQLLKELQ 1089 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0031931; C:TORC1 complex; IDA:UniProtKB.
 GO:0031932; C:TORC2 complex; IDA:UniProtKB.
 GO:0032006; P:regulation of TOR signaling cascade; IMP:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR016441; UCP005250. 
Pfam
  
SMART
  
PROSITE
  
PRINTS