UniProt Accession

 VPS4B_HUMAN; O75351; Q69HW4; Q9GZS7 

Genbank Protein ID

 AF195514; AF282904; AF038960; AY232629; CH471096; BC039574 

Genbank Nucleotide ID

 AAG33022.1; AAG01471.1; AAC39874.1; AAP59551.1; EAW63143.1; AAH39574.1 

Protein Name

 Vacuolar protein sorting-associated protein 4B 

Protein Synonyms/Alias

 Cell migration-inducing gene 1 protein; Suppressor of K(+) transport growth defect 1; Protein SKD1 

Gene Name

 VPS4B 

Gene Synonyms/Alias

 SKD1; VPS42; MIG1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
147	EGAKEALKEAVILPI	ubiquitination	[1, 2, 3]
155	EAVILPIKFPHLFTG	ubiquitination	[1, 2, 3]
163	FPHLFTGKRTPWRGI	ubiquitination	[1, 3]
180	FGPPGTGKSYLAKAV	ubiquitination	[1, 2, 3]
185	TGKSYLAKAVATEAN	ubiquitination	[2]
207	SSSDLVSKWLGESEK	ubiquitination	[2]
217	GESEKLVKNLFQLAR	ubiquitination	[1, 2, 3]
432	VNEHDLLKLKKFTED	acetylation	[4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). 

Sequence Annotation

 DOMAIN 4 82 MIT.
 NP_BIND 174 181 ATP (Potential).
 MOD_RES 93 93 Phosphoserine.
 MOD_RES 102 102 Phosphoserine.
 MOD_RES 108 108 Phosphoserine.  

Keyword

 3D-structure; ATP-binding; Cell cycle; Cell division; Coiled coil; Complete proteome; Endosome; Hydrolase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 444 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0010008; C:endosome membrane; IDA:UniProtKB.
 GO:0005770; C:late endosome; IDA:UniProtKB.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005774; C:vacuolar membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IMP:UniProtKB.
 GO:0042623; F:ATPase activity, coupled; NAS:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0007032; P:endosome organization; IEA:Compara.
 GO:0032510; P:endosome to lysosome transport via multivesicular body sorting pathway; IMP:UniProtKB.
 GO:0032367; P:intracellular cholesterol transport; IMP:UniProtKB.
 GO:0006813; P:potassium ion transport; IEA:Compara.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0033993; P:response to lipid; IDA:UniProtKB. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR007330; MIT.
 IPR027417; P-loop_NTPase.
 IPR015415; Vps4_C. 

Pfam

 PF00004; AAA
 PF04212; MIT
 PF09336; Vps4_C 

SMART

 SM00382; AAA
 SM00745; MIT 

PROSITE

 PS00674; AAA 

PRINTS