CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018787
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GPI-anchor transamidase 
Protein Synonyms/Alias
 GPI transamidase; GPI8 homolog; hGPI8; Phosphatidylinositol-glycan biosynthesis class K protein; PIG-K 
Gene Name
 PIGK 
Gene Synonyms/Alias
 GPI8 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
193AFEQMWQKRRYNELLubiquitination[1, 2]
296DLFQRDPKNVLITDFubiquitination[2, 3, 4, 5]
331EIMESSYKEDQMDEKubiquitination[1, 2]
358VAQIIHQKPKLKDWHubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein. 
Sequence Annotation
 REGION 311 321 Essential for GPI attachment.
 ACT_SITE 164 164
 ACT_SITE 206 206
 DISULFID 92 92 Interchain (with C-182 in PIGT).  
Keyword
 Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; GPI-anchor biosynthesis; Hydrolase; Membrane; Polymorphism; Protease; Reference proteome; Signal; Thiol protease; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 395 AA 
Protein Sequence
MAVTDSLSRA ATVLATVLLL SFGSVAASHI EDQAEQFFRS GHTNNWAVLV CTSRFWFNYR 60
HVANTLSVYR SVKRLGIPDS HIVLMLADDM ACNPRNPKPA TVFSHKNMEL NVYGDDVEVD 120
YRSYEVTVEN FLRVLTGRIP PSTPRSKRLL SDDRSNILIY MTGHGGNGFL KFQDSEEITN 180
IELADAFEQM WQKRRYNELL FIIDTCQGAS MYERFYSPNI MALASSQVGE DSLSHQPDPA 240
IGVHLMDRYT FYVLEFLEEI NPASQTNMND LFQVCPKSLC VSTPGHRTDL FQRDPKNVLI 300
TDFFGSVRKV EITTETIKLQ QDSEIMESSY KEDQMDEKLM EPLKYAEQLP VAQIIHQKPK 360
LKDWHPPGGF ILGLWALIIM VFFKTYGIKH MKFIF 395 
Gene Ontology
 GO:0042765; C:GPI-anchor transamidase complex; IMP:UniProtKB.
 GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
 GO:0003923; F:GPI-anchor transamidase activity; IMP:UniProtKB.
 GO:0003756; F:protein disulfide isomerase activity; TAS:UniProtKB.
 GO:0016255; P:attachment of GPI anchor to protein; TAS:UniProtKB.
 GO:0006501; P:C-terminal protein lipidation; TAS:Reactome.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR001096; Peptidase_C13. 
Pfam
 PF01650; Peptidase_C13 
SMART
  
PROSITE
  
PRINTS
 PR00776; HEMOGLOBNASE.