CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021011
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1,5-anhydro-D-fructose reductase 
Protein Synonyms/Alias
 AF reductase; Aldo-keto reductase family 1 member C-like protein 2; Aldo-keto reductase family 1 member E1; Aldo-keto reductase family 1 member E2 
Gene Name
 Akr1e2 
Gene Synonyms/Alias
 Akr1cl2; Akr1e1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
164QLERLLDKPGLRVRPubiquitination[1]
193KLIDFCHKRNVSVTAubiquitination[1]
279EELLSLDKNLRLATFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Catalyzes the NADPH-dependent reduction of 1,5-anhydro- D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones. 
Sequence Annotation
 NP_BIND 250 258 NADP (By similarity).
 ACT_SITE 40 40 Proton donor (By similarity).
 BINDING 35 35 NADP (By similarity).
 BINDING 102 102 Substrate (By similarity).
 BINDING 174 174 NADP (By similarity).  
Keyword
 Complete proteome; Cytoplasm; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 301 AA 
Protein Sequence
MENIPTVGLG TWKASPGEVT DAVKLAINLG YRHFDCAYLY HNESEVGMGI SEKIKEGVVK 60
REDLFVVSKL WCTCHKKSLV KTACTNTLEA LNLDYLDLYL IHWPMGFKPG EKDIPLDRNG 120
KVIPSHTSFL DTWEAMEDLV FEGLVKNLGV SNFNHEQLER LLDKPGLRVR PITNQIECHP 180
YLNQKKLIDF CHKRNVSVTA YRPLGGSGGG FHLMDDTVIR KIAKKHGKSP AQILIRFQIQ 240
RNLIVIPKSV TPSRIRENIQ VFDFELTEKD MEELLSLDKN LRLATFPTTE NHQDYPFHIE 300
Y 301 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0050571; F:1,5-anhydro-D-fructose reductase activity; IEA:EC. 
Interpro
 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 
Pfam
 PF00248; Aldo_ket_red 
SMART
  
PROSITE
 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 
PRINTS
 PR00069; ALDKETRDTASE.