CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039970
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Serine/threonine-protein kinase MRCK alpha 
Protein Synonyms/Alias
  
Gene Name
 CDC42BPA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
315TDVSENAKDLIRRLIubiquitination[1]
338QNGIEDFKKHPFFSGubiquitination[2]
696LKTDLEKKSIFYEEEubiquitination[3]
1089LYDIAEGKASQPSVVubiquitination[1, 2]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1699 AA 
Protein Sequence
MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 60
KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 120
CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF 180
YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 240
PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 300
FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 360
EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 420
GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQYSTVDGP 480
LTASKDLEIK NLKEEIEKLR KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL 540
QQEREDLNKE LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV 600
RDKEEEVDLV MQKVESLRQE LRRTERAKKE LEVHTEALAA EASKDRKLRE QSEHYSKQLE 660
NELEGLKQKQ ISYSPGVCSI EHQQEITKLK TDLEKKSIFY EEELSKREGI HANEIKNLKK 720
ELHDSEGQQL ALNKEIMILK DKLEKTRRES QSEREEFESE FKQQYEREKV LLTEENKKLT 780
SELDKLTTLY ENLSIHNQQL EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA 840
LASKMTEELE ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL 900
NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKASKGRRTV DSTPLSVHTP 960
TLRKKGCPGS TGFPPKRKTH QFFVKSFTTP TKCHQCTSLM VGLIRQGCSC EVCGFSCHIT 1020
CVNKAPTTCP VPPEQTKGPL GIDPQKGIGT AYEGHVRIPK PAGVKKGWQR ALAIVCDFKL 1080
FLYDIAEGKA SQPSVVISQV IDMRDEEFSV SSVLASDVIH ASRKDIPCIF RVTASQLSAS 1140
NNKCSILMLA DTENEKNKWV GVLSELHKIL KKNKFRDRSV YVPKEAYDST LPLIKTTQAA 1200
AIIDHERIAL GNEEGLFVVH VTKDEIIRVG DNKKIHQIEL IPNDQLVAVI SGRNRHVRLF 1260
PMSALDGRET DFYKLSETKG CQTVTSGKVR HGALTCLCVA MKRQVLCYEL FQSKTRHRKF 1320
KEIQVPYNVQ WMAIFSEQLC VGFQSGFLRY PLNGEGNPYS MLHSNDHTLS FIAHQPMDAI 1380
CAVEISSKEY LLCFNSIGIY TDCQGRRSRQ QELMWPANPS SCCYNAPYLS VYSENAVDIF 1440
DVNSMEWIQT LPLKKVRPLN NEGSLNLLGL ETIRLIYFKN KMAEGDELVV PETSDNSRKQ 1500
MVRNINNKRR YSFRVPEEER MQQRREMLRD PEMRNKLISN PTNFNHIAHM GPGDGIQILK 1560
DLPMNPRPQE SRTVFSGSVS IPSITKSRPE PGRSMSASSG LSARSSAQNG SALKREFSGG 1620
SYSAKRQPMP SPSEGSLSSG GMDQGSDAPA RDFDGEDSDS PRHSTASNSS NLSSPPSPAS 1680
PRKTKSLSLE STDRGSWDP 1699 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0005083; F:small GTPase regulator activity; IEA:InterPro.
 GO:0031532; P:actin cytoskeleton reorganization; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR001180; Citron.
 IPR011009; Kinase-like_dom.
 IPR014930; Myotonic_dystrophy_kinase_coil.
 IPR000095; PAK_box_Rho-bd.
 IPR017892; Pkinase_C.
 IPR001849; Pleckstrin_homology.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR026611; Ser/Thr_kinase_MRCK. 
Pfam
 PF00130; C1_1
 PF00780; CNH
 PF08826; DMPK_coil
 PF00069; Pkinase
 PF00433; Pkinase_C 
SMART
 SM00109; C1
 SM00036; CNH
 SM00285; PBD
 SM00233; PH
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS50219; CNH
 PS50108; CRIB
 PS50003; PH_DOMAIN
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS