UniProt Accession

 GUDD_ECOLI; P0AES2; P76637; P78217; Q46914 

Genbank Protein ID

 U00096; U29581; AP009048 

Genbank Nucleotide ID

 AAC75829.1; AAB40437.1; BAA16572.2 

Protein Name

 Glucarate dehydratase 

Protein Synonyms/Alias

 GDH; GlucD 

Gene Name

 gudD 

Gene Synonyms/Alias

 ygcX; b2787; JW2758 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
60	GEIPGGEKIRKTLED	acetylation	[1]
198	LAEAAYEKYGFNDFK	acetylation	[1]
416	KAHELYQKHGLGARD	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate. 

Sequence Annotation

 REGION 235 237 Substrate binding.
 REGION 339 341 Substrate binding.
 ACT_SITE 207 207 Proton acceptor.
 ACT_SITE 339 339 Proton acceptor.
 METAL 235 235 Magnesium.
 METAL 266 266 Magnesium.
 METAL 289 289 Magnesium.
 BINDING 32 32 Substrate.
 BINDING 103 103 Substrate.
 BINDING 150 150 Substrate.
 BINDING 205 205 Substrate.
 BINDING 289 289 Substrate.
 BINDING 368 368 Substrate.
 BINDING 422 422 Substrate.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Lyase; Magnesium; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 446 AA 

Protein Sequence

Gene Ontology

 GO:0008872; F:glucarate dehydratase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0042838; P:D-glucarate catabolic process; IDA:EcoCyc. 

Interpro

 IPR017653; Glucarate_dehydratase.
 IPR013342; Mandelate_racemase_C.
 IPR001354; MR_MLE. 

Pfam

 PF01188; MR_MLE 

SMART

 SM00922; MR_MLE 

PROSITE

  

PRINTS