CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013879
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S-adenosylmethionine synthase 
Protein Synonyms/Alias
 AdoMet synthase; MAT; Methionine adenosyltransferase 
Gene Name
 metK 
Gene Synonyms/Alias
 TTHA1642 
Created Date
 July 27, 2013 
Organism
 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 
NCBI Taxa ID
 300852 
Lysine Modification
Position
Peptide
Type
References
37DALIAQDKKARVAAEacetylation[1]
Reference
 [1] Acetylome with structural mapping reveals the significance of lysine acetylation in Thermus thermophilus.
 Okanishi H, Kim K, Masui R, Kuramitsu S.
 J Proteome Res. 2013 Aug 1;. [PMID: 23901841] 
Functional Description
 Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme (By similarity). 
Sequence Annotation
 NP_BIND 272 279 ATP (Potential).
 METAL 18 18 Magnesium (By similarity).
 METAL 44 44 Potassium (By similarity).
 METAL 276 276 Potassium (By similarity).
 METAL 284 284 Magnesium (By similarity).  
Keyword
 ATP-binding; Cobalt; Complete proteome; Cytoplasm; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 395 AA 
Protein Sequence
MRALRLVTSE SVTEGHPDKL ADRISDAILD ALIAQDKKAR VAAETLVTTG LVFVAGEITT 60
EGYVDIPNLV RKTVREVGYT RAKYGFDADT CAVLTAIDEQ SPDIAGGVNL SYEWRVLKST 120
DPLDRVGAGD QGLMFGYATD ETPELMPLPI TLAHRLTMRL AEVRKTGLLP YLRPDGKAQV 180
TVVYEGDKPL YVKTVVVSAQ HSPEVEQEQL REDLIREVVR QAIPPEYLKD GETEYLINPS 240
GRFILGGPHA DTGLTGRKII VDTYGGAVPH GGGAFSGKDP TKVDRSASYY ARYMAKNIVA 300
AGLARRALVE LAYAIGKARP VSLRVETFGT GVLPDEKLTE IAKKVFDPRP LAIIEELDLL 360
RPIYTPTSAY GHFGRPGFPW EETDRVEALR REAGL 395 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0004478; F:methionine adenosyltransferase activity; IEA:HAMAP.
 GO:0006730; P:one-carbon metabolic process; IEA:HAMAP.
 GO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR022631; ADOMET_SYNTHASE_CS.
 IPR022630; S-AdoMet_synt_C.
 IPR022629; S-AdoMet_synt_central.
 IPR022628; S-AdoMet_synt_N.
 IPR002133; S-AdoMet_synthetase.
 IPR022636; S-AdoMet_synthetase_sfam. 
Pfam
 PF02773; S-AdoMet_synt_C
 PF02772; S-AdoMet_synt_M
 PF00438; S-AdoMet_synt_N 
SMART
  
PROSITE
 PS00376; ADOMET_SYNTHASE_1
 PS00377; ADOMET_SYNTHASE_2 
PRINTS