CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022004
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor 7-like 2 
Protein Synonyms/Alias
 HMG box transcription factor 4; T-cell-specific transcription factor 4; T-cell factor 4; TCF-4; hTCF-4 
Gene Name
 TCF7L2 
Gene Synonyms/Alias
 TCF4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45ERDLADVKSSLVNESubiquitination[1]
88ESLEEAAKRQDGGLFubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. 
Sequence Annotation
 DNA_BIND 350 418 HMG box.
 REGION 1 53 CTNNB1-binding (By similarity).
 REGION 201 395 Mediates interaction with MAD2L2.
 REGION 459 505 Promoter-specific activation domain.
 MOTIF 425 430 Nuclear localization signal (Potential).
 MOD_RES 201 201 Phosphothreonine; by NLK (Probable).
 MOD_RES 212 212 Phosphothreonine; by NLK (Probable).
 CROSSLNK 320 320 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; Diabetes mellitus; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 619 AA 
Protein Sequence
MPQLNGGGGD DLGANDELIS FKDEGEQEEK SSENSSAERD LADVKSSLVN ESETNQNSSS 60
DSEAERRPPP RSESFRDKSR ESLEEAAKRQ DGGLFKGPPY PGYPFIMIPD LTSPYLPNGS 120
LSPTARTYLQ MKWPLLDVQA GSLQSRQALK DARSPSPAHI VSNKVPVVQH PHHVHPLTPL 180
ITYSNEHFTP GNPPPHLPAD VDPKTGIPRP PHPPDISPYY PLSPGTVGQI PHPLGWQGQP 240
VYPITTGGFR HPYPTALTVN ASMSRFPPHM VPPHHTLHTT GIPHPAIVTP TVKQESSQSD 300
VGSLHSSKHQ DSKKEEEKKK PHIKKPLNAF MLYMKEMRAK VVAECTLKES AAINQILGRR 360
WHALSREEQA KYYELARKER QLHMQLYPGW SARDNYGKKK KRKRDKQPGE TNEHSECFLN 420
PCLSLPPITG EKKSAFATYK VKAAASAHPL QMEAY 455 
Gene Ontology
 GO:0070369; C:beta-catenin-TCF7L2 complex; IDA:BHF-UCL.
 GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0032993; C:protein-DNA complex; IDA:BHF-UCL.
 GO:0005667; C:transcription factor complex; IBA:RefGenome.
 GO:0008013; F:beta-catenin binding; IDA:BHF-UCL.
 GO:0003682; F:chromatin binding; IBA:RefGenome.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0001568; P:blood vessel development; IMP:BHF-UCL.
 GO:0030282; P:bone mineralization; IEA:Compara.
 GO:0007420; P:brain development; IBA:RefGenome.
 GO:0044334; P:canonical Wnt receptor signaling pathway involved in positive regulation of epithelial to mesenchymal transition; IMP:BHF-UCL.
 GO:0035411; P:catenin import into nucleus; IEA:Compara.
 GO:0007050; P:cell cycle arrest; IMP:BHF-UCL.
 GO:0008283; P:cell proliferation; IMP:BHF-UCL.
 GO:0048557; P:embryonic digestive tract morphogenesis; IEA:Compara.
 GO:0030538; P:embryonic genitalia morphogenesis; IEA:Compara.
 GO:0048619; P:embryonic hindgut morphogenesis; IEA:Compara.
 GO:0060325; P:face morphogenesis; IEA:Compara.
 GO:0045444; P:fat cell differentiation; IDA:BHF-UCL.
 GO:0048699; P:generation of neurons; IBA:RefGenome.
 GO:0042593; P:glucose homeostasis; IDA:BHF-UCL.
 GO:0043570; P:maintenance of DNA repeat elements; IMP:BHF-UCL.
 GO:0048625; P:myoblast fate commitment; IDA:BHF-UCL.
 GO:0043066; P:negative regulation of apoptotic process; IDA:BHF-UCL.
 GO:0030514; P:negative regulation of BMP signaling pathway; IEA:Compara.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IMP:UniProtKB.
 GO:0045599; P:negative regulation of fat cell differentiation; IEA:Compara.
 GO:0040037; P:negative regulation of fibroblast growth factor receptor signaling pathway; IEA:Compara.
 GO:0046621; P:negative regulation of organ growth; IEA:Compara.
 GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0021915; P:neural tube development; IEA:Compara.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Compara.
 GO:0014003; P:oligodendrocyte development; IEA:Compara.
 GO:0031016; P:pancreas development; TAS:BHF-UCL.
 GO:0021983; P:pituitary gland development; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IEA:Compara.
 GO:0010909; P:positive regulation of heparan sulfate proteoglycan biosynthetic process; IMP:BHF-UCL.
 GO:0032024; P:positive regulation of insulin secretion; IDA:BHF-UCL.
 GO:0032092; P:positive regulation of protein binding; IDA:BHF-UCL.
 GO:0046827; P:positive regulation of protein export from nucleus; IMP:BHF-UCL.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IMP:BHF-UCL.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0032350; P:regulation of hormone metabolic process; IDA:BHF-UCL.
 GO:0061178; P:regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Compara.
 GO:0031641; P:regulation of myelination; IEA:Compara.
 GO:0048713; P:regulation of oligodendrocyte differentiation; IEA:Compara.
 GO:0048641; P:regulation of skeletal muscle tissue development; IEA:Compara.
 GO:0048660; P:regulation of smooth muscle cell proliferation; IMP:BHF-UCL.
 GO:0009749; P:response to glucose stimulus; ISS:BHF-UCL.
 GO:0032252; P:secretory granule localization; IEA:Compara.
 GO:0043588; P:skin development; IEA:Compara.
 GO:0035019; P:somatic stem cell maintenance; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR027397; Catenin_binding_dom.
 IPR013558; CTNNB1-bd_N.
 IPR009071; HMG_box_dom.
 IPR024940; TCF/LEF. 
Pfam
 PF08347; CTNNB1_binding
 PF00505; HMG_box 
SMART
 SM00398; HMG 
PROSITE
 PS50118; HMG_BOX_2 
PRINTS