CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039637
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Methionine aminopeptidase 
Protein Synonyms/Alias
  
Gene Name
 METAP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73SESEQALKGTSQIKLubiquitination[1]
79LKGTSQIKLLSSEDIubiquitination[1, 2, 3]
108DVAAGMIKPGVTTEEubiquitination[4]
141LNYYNFPKSCCTSVNubiquitination[3]
228ELGNIIQKHAQANGFubiquitination[1, 2, 4]
261PNVPHYAKNKAVGVMubiquitination[1, 2]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Removes the N-terminal methionine from nascent proteins (By similarity). 
Sequence Annotation
  
Keyword
 Aminopeptidase; Cobalt; Complete proteome; Hydrolase; Metal-binding; Protease; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 336 AA 
Protein Sequence
MCTMHKDEKA KREVSSWTVE GDINTDPWAG YRYTGKLRPH YPLMPTRPVP SYIQRPDYAD 60
HPLGMSESEQ ALKGTSQIKL LSSEDIEGMR LVCRLAREVL DVAAGMIKPG VTTEEIDHAV 120
HLACIARNCY PSPLNYYNFP KSCCTSVNEV ICHGIPDRRP LQEGDIVNVD ITLYRNGYHG 180
DLNETFFVGE VDDGARKLVQ TTYECLMQAI DAVKPGVRYR ELGNIIQKHA QANGFSVVRS 240
YCGHGIHKLF HTAPNVPHYA KNKAVGVMKS GHVFTIEPMI CEGGWQDETW PDGWTAVTRD 300
GKRSAQFEHT LLVTDTGCEI LTRRLDSARP HFMSQF 336 
Gene Ontology
 GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008235; F:metalloexopeptidase activity; IEA:InterPro.
 GO:0009987; P:cellular process; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR001714; Pept_M24_MAP.
 IPR000994; Pept_M24_structural-domain.
 IPR002467; Pept_M24A_MAP1. 
Pfam
 PF00557; Peptidase_M24 
SMART
  
PROSITE
 PS00680; MAP_1 
PRINTS
 PR00599; MAPEPTIDASE.