UniProt Accession

 RAC1_MOUSE; P63001; O95501; P15154; Q9BTB4 

Genbank Protein ID

 X57277; AK009017; AK011072; AK034601; AK047969; AK088825; BC003828; BC051053 

Genbank Nucleotide ID

 CAA40545.1; BAB26027.1; BAB69451.1; BAC28767.1; BAC33203.1; BAC40596.1; AAH03828.1; AAH51053.1 

Protein Name

 Ras-related C3 botulinum toxin substrate 1 

Protein Synonyms/Alias

 p21-Rac1 

Gene Name

 Rac1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
116	PIILVGTKLDLRDDK	ubiquitination	[1]
123	KLDLRDDKDTIEKLK	acetylation	[2, 3]
123	KLDLRDDKDTIEKLK	ubiquitination	[1]
128	DDKDTIEKLKEKKLT	ubiquitination	[1]
133	IEKLKEKKLTPITYP	acetylation	[4]
133	IEKLKEKKLTPITYP	ubiquitination	[1]
147	PQGLAMAKEIGAVKY	ubiquitination	[1]
153	AKEIGAVKYLECSAL	acetylation	[4]
153	AKEIGAVKYLECSAL	ubiquitination	[1]
183	VLCPPPVKKRKRKCL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion (By similarity). 

Sequence Annotation

 NP_BIND 10 17 GTP (By similarity).
 NP_BIND 57 61 GTP (By similarity).
 NP_BIND 115 118 GTP (By similarity).
 MOTIF 32 40 Effector region (Potential).
 MOD_RES 189 189 Cysteine methyl ester (By similarity).
 LIPID 189 189 S-geranylgeranyl cysteine (By
 CROSSLNK 147 147 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Cell membrane; Complete proteome; Cytoplasm; GTP-binding; Isopeptide bond; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 192 AA 

Protein Sequence

Gene Ontology

 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IDA:MGI.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0019897; C:extrinsic to plasma membrane; IDA:MGI.
 GO:0000139; C:Golgi membrane; IEA:Compara.
 GO:0030027; C:lamellipodium; IDA:MGI.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0001891; C:phagocytic cup; IDA:UniProtKB.
 GO:0032587; C:ruffle membrane; IDA:UniProtKB.
 GO:0005802; C:trans-Golgi network; IEA:Compara.
 GO:0005525; F:GTP binding; IDA:MGI.
 GO:0003924; F:GTPase activity; IDA:MGI.
 GO:0007015; P:actin filament organization; IEA:Compara.
 GO:0048532; P:anatomical structure arrangement; IMP:MGI.
 GO:0002093; P:auditory receptor cell morphogenesis; IMP:MGI.
 GO:0007411; P:axon guidance; IMP:MGI.
 GO:0045453; P:bone resorption; IEA:Compara.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0045216; P:cell-cell junction organization; IMP:MGI.
 GO:0021799; P:cerebral cortex radially oriented cell migration; IMP:MGI.
 GO:0090103; P:cochlea morphogenesis; IMP:MGI.
 GO:0007010; P:cytoskeleton organization; IDA:MGI.
 GO:0048813; P:dendrite morphogenesis; IGI:MGI.
 GO:0071542; P:dopaminergic neuron differentiation; IGI:MGI.
 GO:0021831; P:embryonic olfactory bulb interneuron precursor migration; IMP:MGI.
 GO:0043652; P:engulfment of apoptotic cell; IDA:BHF-UCL.
 GO:0003382; P:epithelial cell morphogenesis; IMP:MGI.
 GO:0006972; P:hyperosmotic response; IDA:MGI.
 GO:0030032; P:lamellipodium assembly; IDA:MGI.
 GO:0051668; P:localization within membrane; IEA:Compara.
 GO:0002551; P:mast cell chemotaxis; IEA:Compara.
 GO:0032707; P:negative regulation of interleukin-23 production; IEA:Compara.
 GO:0030838; P:positive regulation of actin filament polymerization; IDA:MGI.
 GO:0045740; P:positive regulation of DNA replication; IEA:Compara.
 GO:0010592; P:positive regulation of lamellipodium assembly; ISO:MGI.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IMP:MGI.
 GO:0030334; P:regulation of cell migration; IMP:MGI.
 GO:0060263; P:regulation of respiratory burst; IEA:Compara.
 GO:0097178; P:ruffle assembly; IMP:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IDA:MGI.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IDA:MGI.
 GO:0060071; P:Wnt receptor signaling pathway, planar cell polarity pathway; IGI:MGI. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 

Pfam

 PF00071; Ras 

SMART

 SM00174; RHO 

PROSITE

 PS51420; RHO 

PRINTS

 PR00449; RASTRNSFRMNG.