CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010105
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Leucine--tRNA ligase 
Protein Synonyms/Alias
 Leucyl-tRNA synthetase; LeuRS 
Gene Name
 leuS 
Gene Synonyms/Alias
 MPN_384; MP453 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
462WRNQEWAKVKQGNQTacetylation[1]
759VDEQTVLKAFKTDPEacetylation[1]
791RIINVLLKSE*****acetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
  
Sequence Annotation
 MOTIF 39 50 "HIGH" region.
 MOTIF 569 573 "KMSKS" region.
 BINDING 572 572 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 793 AA 
Protein Sequence
MYNHNLLEEK WLQLWKTKQV NRFFDDKTKP KYYILDMFPY PSAAGLHLGH VRAYTITDVL 60
SRYHKAKGFN VIHPIGFDAF GLPAEQYAIA SNKHPGDWTD KNITNFIEQL TAFGFDYDYQ 120
LSLKTTDPRY YQYTQWIFGQ LFEAGLAEVK EIDVNWCAEL GTVLANEEVL IDSNGNAVSE 180
RGEFPVTKRK MKQWVLKITA FAESLLNGLA ELDWHQSIKE MQTNWIGKSE GVEVTFDIEN 240
SKETITIFTT KVQTIFGVTF LALSNSHPLV KEVAKTNPKI AQFLQKQAQK TTTVKQPETL 300
HDGVDLKLKA INPATNTAIP LYVANYVVEG YGTDAVMGVP AHNENDNFFA RKQKLPIITV 360
IDKQERLQHS GQFSGLNSQT ANTQITQMLV ERQKAKKTTV YKLRDWIFSR QRYWGEPFPI 420
LFDENNQPHL VKELPVTLPA LANYQPDGST NPPLWRNQEW AKVKQGNQTF TRETSTMPQW 480
AGSCWYYLGY LMLINNENFW PIDSREAKDL FERYLPVDLY VGGAEHAVLH LLYARFWHQF 540
LYQKGIVTTK EPFKKLINQG MVLGPDGKKM SKSKGNTINP TPLIDSHGAD ALRLYLMFMG 600
PISAALTWLD DGLNGMRRWL DRVHNFFHKE NIIKETVDQE TVYGYNLFLK RSFEHLEKQE 660
LNLVISQMMI FLNLLYKTKQ LTLAYAKGFL TVLSFFAPYL AEELNAKLGM EPFIVHAQLP 720
NVDNSVLETD KVKIILSVNG RFKGTKEFAK GVDEQTVLKA FKTDPEFQAL FDQPLARVVF 780
VPNRIINVLL KSE 793 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004823; F:leucine-tRNA ligase activity; IEA:HAMAP.
 GO:0006429; P:leucyl-tRNA aminoacylation; IEA:HAMAP.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002300; aa-tRNA-synth_Ia.
 IPR002302; Leu-tRNA-ligase_bac/mito.
 IPR025709; Leu_tRNA-synth_edit.
 IPR015413; Methionyl/Leucyl_tRNA_Synth.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd.
 IPR009008; Val/Leu/Ile-tRNA-synth_edit. 
Pfam
 PF00133; tRNA-synt_1
 PF13603; tRNA-synt_1_2
 PF09334; tRNA-synt_1g 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00985; TRNASYNTHLEU.