UniProt Accession

 NDUS3_HUMAN; O75489; B2R9J1; Q9UNQ8 

Genbank Protein ID

 AF067139; AF200954; AF100743; AK313802; CH471064; BC000617 

Genbank Nucleotide ID

 AAC27451.1; AAG17541.1; AAD40386.1; BAG36538.1; EAW67895.1; AAH00617.1 

Protein Name

 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial 

Protein Synonyms/Alias

 Complex I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit 

Gene Name

 NDUFS3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
56	PRNDVAHKQLSAFGE	ubiquitination	[1, 2, 3, 4]
109	DHTNAQFKSLVDLTA	ubiquitination	[3]
144	FNSRIRVKTYTDELT	ubiquitination	[2, 3]
200	FEGHPFRKDFPLSGY	ubiquitination	[1, 2, 3, 4, 5]
232	ELAQEFRKFDLNSPW	ubiquitination	[1, 2, 3, 4, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). 

Sequence Annotation

  

Keyword

 Complete proteome; Direct protein sequencing; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Polymorphism; Reference proteome; Respiratory chain; Transit peptide; Transport; Ubiquinone. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 264 AA 

Protein Sequence

Gene Ontology

 GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
 GO:0009055; F:electron carrier activity; NAS:UniProtKB.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; NAS:UniProtKB.
 GO:0006917; P:induction of apoptosis; IMP:UniProtKB.
 GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; NAS:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; IMP:UniProtKB.
 GO:0072593; P:reactive oxygen species metabolic process; IMP:UniProtKB.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR010218; NADH_DH_suC.
 IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
 IPR020396; NADH_UbQ_OxRdtase_CS. 

Pfam

 PF00329; Complex1_30kDa 

SMART

  

PROSITE

 PS00542; COMPLEX1_30K 

PRINTS