CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007561
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatidylinositol 4-kinase alpha 
Protein Synonyms/Alias
 PI4-kinase alpha; PI4K-alpha; PtdIns-4-kinase alpha 
Gene Name
 PI4KA 
Gene Synonyms/Alias
 PIK4; PIK4CA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
149MEESLLSKLFPKIPPubiquitination[1]
194VCQEGTLKRKTSSVSubiquitination[2]
196QEGTLKRKTSSVSSIubiquitination[2]
374HNELSPLKLRCQANAubiquitination[1, 2]
411LSEKLQSKTSSKVIIubiquitination[1]
462IPSPVLVKLYKYHSQubiquitination[1, 2]
498LNVMSGKKSQPSMYEubiquitination[2]
549YISQESDKDAHLIPDubiquitination[2]
574VALRDTPKVMEPILQubiquitination[2]
701SEKGPALKASSSAGNubiquitination[2]
780PLLTFPSKEPLRSVLubiquitination[2]
794LQYNSAMKNDTVTPAubiquitination[1, 2]
930RIRRVADKYLSGLVDacetylation[3]
997EARESIVKDFAARCGubiquitination[2]
1012MILQEAMKWAPTVTKubiquitination[2]
1019KWAPTVTKSHLQEYLubiquitination[2, 4, 5]
1075SERPACVKKDYSNFMubiquitination[2]
1076ERPACVKKDYSNFMAubiquitination[2]
1113GQMSDLNKMMVQDLHubiquitination[2]
1278SLNIGGAKGSMNRHVubiquitination[1, 2]
1473SVANWRSKYISLSEKubiquitination[2]
1505VQLPARFKNTEAIGNubiquitination[2]
1691TNVSAIIKPYPKGDEubiquitination[1, 4, 5]
1742TPMQSAAKAPYLAKFubiquitination[2]
1748AKAPYLAKFKVKRCGubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate. 
Sequence Annotation
 DOMAIN 1472 1660 PIK helical.
 DOMAIN 1788 2021 PI3K/PI4K.
 REGION 1661 1793 Pleckstrin homology (PH) domain
 MOD_RES 199 199 Phosphoserine.
 MOD_RES 202 202 Phosphoserine.
 MOD_RES 204 204 Phosphoserine.
 MOD_RES 207 207 Phosphoserine.
 MOD_RES 1378 1378 Phosphoserine.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2044 AA 
Protein Sequence
MCPVDFHGIF QLDERRRDAV IALGIFLIES DLQHKDCVVP YLLRLLKGLP KVYWVEESTA 60
RKGRGALPVA ESFSFCLVTL LSDVAYRDPS LRDEILEVLL QVLHVLLGMC QALEIQDKEY 120
LCKYAIPCLI GISRAFGRYS NMEESLLSKL FPKIPPHSLR VLEELEGVRR RSFNDFRSIL 180
PSNLLTVCQE GTLKRKTSSV SSISQVSPER GMPPPSSPGG SAFHYFEASC LPDGTALEPE 240
YYFSTISSSF SVSPLFNGVT YKEFNIPLEM LRELLNLVKK IVEEAVLKSL DAIVASVMEA 300
NPSADLYYTS FSDPLYLTMF KMLRDTLYYM KDLPTSFVKE IHDFVLEQFN TSQGELQKIL 360
HDADRIHNEL SPLKLRCQAN AACVDLMVWA VKDEQGAENL CIKLSEKLQS KTSSKVIIAH 420
LPLLICCLQG LGRLCERFPV VVHSVTPSLR DFLVIPSPVL VKLYKYHSQY HTVAGNDIKI 480
SVTNEHSEST LNVMSGKKSQ PSMYEQLRDI AIDNICRCLK AGLTVDPVIV EAFLASLSNR 540
LYISQESDKD AHLIPDHTIR ALGHIAVALR DTPKVMEPIL QILQQKFCQP PSPLDVLIID 600
QLGCLVITGN QYIYQEVWNL FQQISVKASS VVYSATKDYK DHGYRHCSLA VINALANIAA 660
NIQDEHLVDE LLMNLLELFV QLGLEGKRAS ERASEKGPAL KASSSAGNLG VLIPVIAVLT 720
RRLPPIKEAK PRLQKLFRDF WLYSVLMGFA VEGSGLWPEE WYEGVCEIAT KSPLLTFPSK 780
EPLRSVLQYN SAMKNDTVTP AELSELRSTI INLLDPPPEV SALINKLDFA MSTYLLSVYR 840
LEYMRVLRST DPDRFQVMFC YFEDKAIQKD KSGMMQCVIA VADKVFDAFL NMMADKAKTK 900
ENEEELERHA QFLLVNFNHI HKRIRRVADK YLSGLVDKFP HLLWSGTVLK TMLDILQTLS 960
LSLSADIHKD QPYYDIPDAP YRITVPDTYE ARESIVKDFA ARCGMILQEA MKWAPTVTKS 1020
HLQEYLNKHQ NWVSGLSQHT GLAMATESIL HFAGYNKQNT TLGATQLSER PACVKKDYSN 1080
FMASLNLRNR YAGEVYGMIR FSGTTGQMSD LNKMMVQDLH SALDRSHPQH YTQAMFKLTA 1140
MLISSKDCDP QLLHHLCWGP LRMFNEHGME TALACWEWLL AGKDGVEVPF MREMAGAWHM 1200
TVEQKFGLFS AEIKEADPLA ASEASQPKPC PPEVTPHYIW IDFLVQRFEI AKYCSSDQVE 1260
IFSSLLQRSM SLNIGGAKGS MNRHVAAIGP RFKLLTLGLS LLHADVVPNA TIRNVLREKI 1320
YSTAFDYFSC PPKFPTQGEK RLREDISIMI KFWTAMFSDK KYLTASQLVP PDNQDTRSNL 1380
DITVGSRQQA TQGWINTYPL SSGMSTISKK SGMSKKTNRG SQLHKYYMKR RTLLLSLLAT 1440
EIERLITWYN PLSAPELELD QAGENSVANW RSKYISLSEK QWKDNVNLAW SISPYLAVQL 1500
PARFKNTEAI GNEVTRLVRL DPGAVSDVPE AIKFLVTWHT IDADAPELSH VLCWAPTDPP 1560
TGLSYFSSMY PPHPLTAQYG VKVLRSFPPD AILFYIPQIV QALRYDKMGY VREYILWAAS 1620
KSQLLAHQFI WNMKTNIYLD EEGHQKDPDI GDLLDQLVEE ITGSLSGPAK DFYQREFDFF 1680
NKITNVSAII KPYPKGDERK KACLSALSEV KVQPGCYLPS NPEAIVLDID YKSGTPMQSA 1740
AKAPYLAKFK VKRCGVSELE KEGLRCRSDS EDECSTQEAD GQKISWQAAI FKVGDDCRQD 1800
MLALQIIDLF KNIFQLVGLD LFVFPYRVVA TAPGCGVIEC IPDCTSRDQL GRQTDFGMYD 1860
YFTRQYGDES TLAFQQARYN FIRSMAAYSL LLFLLQIKDR HNGNIMLDKK GHIIHIDFGF 1920
MFESSPGGNL GWEPDIKLTD EMVMIMGGKM EATPFKWFME MCVRGYLAVR PYMDAVVSLV 1980
TLMLDTGLPC FRGQTIKLLK HRFSPNMTER EAANFIMKVI QSCFLSNRSR TYDMIQYYQN 2040
DIPY 2044 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005798; C:Golgi-associated vesicle; TAS:ProtInc.
 GO:0005886; C:plasma membrane; IBA:RefGenome.
 GO:0004430; F:1-phosphatidylinositol 4-kinase activity; IBA:RefGenome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:InterPro.
 GO:0007165; P:signal transduction; NAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0007268; P:synaptic transmission; TAS:ProtInc. 
Interpro
 IPR016024; ARM-type_fold.
 IPR011009; Kinase-like_dom.
 IPR000403; PI3/4_kinase_cat_dom.
 IPR018936; PI3/4_kinase_CS.
 IPR015433; PI_Kinase.
 IPR001263; PInositide-3_kin_accessory_dom. 
Pfam
 PF00454; PI3_PI4_kinase
 PF00613; PI3Ka 
SMART
 SM00145; PI3Ka
 SM00146; PI3Kc 
PROSITE
 PS00915; PI3_4_KINASE_1
 PS00916; PI3_4_KINASE_2
 PS50290; PI3_4_KINASE_3
 PS51545; PIK_HELICAL 
PRINTS