CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011027
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Down-regulator of invasive growth 1 
Protein Synonyms/Alias
 Regulator of STE12 protein 1; Regulator of sterile twelve 1 
Gene Name
 DIG1 
Gene Synonyms/Alias
 RST1; YPL049C; P7102.02 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
152AQVTQHSKFQPRVQYacetylation[1]
180NSSNSYGKPHMPSAGubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 DIG1 and DIG2 are negative regulators of the filamentation and pheromone induced mating program. DIG1 and DIG2 inhibit the transcriptional activity of STE12 by direct protein- protein interaction. DIG1 colocalizes to promoters with STE12 and redistributes with it during induction of filamentation (by butanol) or mating (by pheromone) to program specific genes, but binding of DIG1 to STE12 is reduced by pheromone treatment. 
Sequence Annotation
 REGION 212 452 Interaction with FUS3 and KSS1.
 MOD_RES 45 45 Phosphoserine.
 MOD_RES 126 126 Phosphoserine.
 MOD_RES 142 142 Phosphoserine.
 MOD_RES 272 272 Phosphoserine.
 MOD_RES 275 275 Phosphoserine.
 MOD_RES 330 330 Phosphoserine.
 MOD_RES 379 379 Phosphothreonine.
 MOD_RES 428 428 Phosphoserine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 452 AA 
Protein Sequence
MAVSARLRTT AEDTSIAKST QDPIGDTEIS VANAKGSSDS NIKNSPGGNS VGQESELEHV 60
PEEDDSGDKE ADHEDSETAT AKKRKAQPLK NPKKSLKRGR VPAPLNLSDS NTNTHGGNIK 120
DGNLASSNSA HFPPVANQNV KSAPAQVTQH SKFQPRVQYL GKASSRQSIQ VNNSSNSYGK 180
PHMPSAGIMS AMNPYMPMNR YIMSPYYNPY GIPPPHMLNK PIMTPYVSYP YPMGPRTSIP 240
YAMQGGNARP YEENEYSASN YRNKRVNDSY DSPLSGTAST GKTRRSEEGS RNSSVGSSAN 300
AGPTQQRADL RPADMIPAEE YHFERDALLS ANTKARSAST STSTSTSTNR DRSSWHEAEP 360
NKDEEEGTDL AIEDGAVPTP TFTTFQRTSQ PQQQSPSLLQ GEIRLSSHIF AFEFPLSSSN 420
VDKKMFMSIC NKVWNESKEL TKKSSSHHRT GK 452 
Gene Ontology
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0003677; F:DNA binding; IPI:SGD.
 GO:0008134; F:transcription factor binding; IDA:SGD.
 GO:2000218; P:negative regulation of invasive growth in response to glucose limitation; IGI:SGD.
 GO:2000221; P:negative regulation of pseudohyphal growth; IGI:SGD.
 GO:0046020; P:negative regulation of transcription from RNA polymerase II promoter by pheromones; IGI:SGD. 
Interpro
  
Pfam
  
SMART
  
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PRINTS