CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-045377
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Epidermal growth factor receptor substrate 15-like 1 
Protein Synonyms/Alias
  
Gene Name
 EPS15L1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
400IAQLQREKYSLEQDIubiquitination[1]
492QSQESDLKSQEDDLNubiquitination[2, 3, 4, 5]
531VQLETIIKSLKSTQDubiquitination[1, 2, 3, 4, 5, 6, 7]
534ETIIKSLKSTQDEINubiquitination[2, 3, 4, 5]
546EINQARSKLSQLHESubiquitination[1, 4]
606KDDPFKNKALLFSNNubiquitination[2, 3, 5, 6]
629FQTEDPFKSDPFKGAubiquitination[1, 2, 3, 4, 5]
634PFKSDPFKGADPFKGubiquitination[2, 6]
640FKGADPFKGDPFQNDubiquitination[2, 3, 5, 6]
666PFGGDPFKESDPFRGubiquitination[2, 3, 5]
681SATDDFFKKQTKNDPubiquitination[1, 2, 3, 4, 5, 6]
682ATDDFFKKQTKNDPFubiquitination[2, 4]
685DFFKKQTKNDPFTSDubiquitination[1, 2, 3, 4, 5, 6]
696FTSDPFTKNPSLPSKubiquitination[1, 2, 3, 4, 5, 6, 7]
703KNPSLPSKLDPFESSubiquitination[1, 2, 3, 4, 5, 6, 7]
721SSSSVSSKGSDPFGTubiquitination[5]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 756 AA 
Protein Sequence
MAAPLIPLSQ QIPTGNSLYE SYYKQVDPAY TGRVGASEAA LFLKKSGLSD IILGKIWDLA 60
DPEGKGFLDK QGFYVALRLV ACAQSGHEVT LSNLNLSMPP PKFHDTSSPL MVTPPSAEAH 120
WAVRVEEKAK FDGIFESLLP INGLLSGDKV KPVLMNSKLP LDVLGRVWDL SDIDKDGHLD 180
RDEFAVAMHL VYRALEKEPV PSALPPSLIP PSKRKKTVFP GAVPVLPASP PPKDSLRSTP 240
SHGSVSSLNS TGSLSPKHSL KQTQPTVNWV VPVADKMRFD EIFLKTDLDL DGYVSGQEVK 300
EIFMHSGLTQ NLLAHIWALA DTRQTGKLSK DQFALAMYFI QQKVSKGIDP PQVLSPDMVP 360
PSERGTPGPD SSGSLGSGEF TGVKELDDIS QEIAQLQREK YSLEQDIREK EEAIRQKTSE 420
VQELQNDLDR ETSSLQELEA QKQDAQDRLD EMDQQKAKLR DMLSDVRQKC QDETQMISSL 480
KTQIQSQESD LKSQEDDLNR AKSELNRLQQ EETQLEQSIQ AGRVQLETII KSLKSTQDEI 540
NQARSKLSQL HESRQEAHRS LEQYDQVLDG AHGASLTDLA NLSEGVSLAE RGSFGAMVKD 600
DPFKNKALLF SNNTQELHPD PFQTEDPFKS DPFKGADPFK GDPFQNDPFA EQQTTSTDPF 660
GGDPFKESDP FRGSATDDFF KKQTKNDPFT SDPFTKNPSL PSKLDPFESS DPFSSSSVSS 720
KGSDPFGTLD PFGSGSFNSA EGFADFSQMS KVKVHL 756 
Gene Ontology
 GO:0005509; F:calcium ion binding; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology. 
Pfam
  
SMART
 SM00054; EFh
 SM00027; EH 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH 
PRINTS