UniProt Accession

 KAP2_MOUSE; P12367 

Genbank Protein ID

 J02935 

Genbank Nucleotide ID

 AAA39932.1 

Protein Name

 cAMP-dependent protein kinase type II-alpha regulatory subunit 

Protein Synonyms/Alias

  

Gene Name

 Prkar2a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
132	CRLQEACKDILLFKN	acetylation	[1]
158	AMFEKIVKTDEHVID	ubiquitination	[2]
247	KNNAKKRKMFESFIE	ubiquitination	[2]
277	IVDVIGEKIYKDGER	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. 

Sequence Annotation

 NP_BIND 136 257 cAMP 1.
 NP_BIND 258 401 cAMP 2.
 REGION 2 135 Dimerization and phosphorylation.
 BINDING 205 205 cAMP 1.
 BINDING 214 214 cAMP 1.
 BINDING 335 335 cAMP 2.
 BINDING 344 344 cAMP 2.
 MOD_RES 2 2 N-acetylserine (Probable).
 MOD_RES 47 47 Phosphoserine (By similarity).
 MOD_RES 74 74 Phosphoserine (By similarity).
 MOD_RES 76 76 Phosphoserine (By similarity).
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 212 212 Phosphothreonine; by PDPK1 (By  

Keyword

 3D-structure; Acetylation; cAMP; cAMP-binding; Cell membrane; Complete proteome; Cytoplasm; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 401 AA 

Protein Sequence

Gene Ontology

 GO:0005952; C:cAMP-dependent protein kinase complex; IEA:InterPro.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
 GO:0008603; F:cAMP-dependent protein kinase regulator activity; IEA:InterPro.
 GO:0007165; P:signal transduction; IEA:InterPro. 

Interpro

 IPR002373; cAMP/cGMP_kin.
 IPR012198; cAMP_dep_PK_reg_su.
 IPR003117; cAMP_dep_PK_reg_su_I/II_a/b.
 IPR018490; cNMP-bd-like.
 IPR018488; cNMP-bd_CS.
 IPR000595; cNMP-bd_dom.
 IPR014710; RmlC-like_jellyroll. 

Pfam

 PF00027; cNMP_binding
 PF02197; RIIa 

SMART

 SM00100; cNMP
 SM00394; RIIa 

PROSITE

 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3 

PRINTS

 PR00103; CAMPKINASE.