CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006839
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA repair and recombination protein RDH54 
Protein Synonyms/Alias
 RAD homolog 54; Recombination factor TID1; Two hybrid interaction with DMC1 protein 1; DNA topoisomerase; Putative helicase 
Gene Name
 RDH54 
Gene Synonyms/Alias
 TID1; YBR073W; YBR0715 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
55PRRVGSNKYTQLKPTacetylation[1]
218STKNDGGKYQMPLSQacetylation[1]
649PYYKSHIKDTQSQDSubiquitination[2, 3]
811MKNSLSQKFLGDSEMacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Involved in the recombinational repair of double-strand breaks (DSB) in DNA during mitosis and meiosis. Has DNA dependent ATPase activity. Promotes D-loop (displacement loop) formation with RAD51 recombinase. Modifies the topology of double-stranded DNA during the D-loop reaction to facilitate the invasion of the homologous duplex molecule by the initiating single-stranded DNA substrate. Required for adaptation from G2/M checkpoint arrest induced by a double strand break, by participating in monitoring the extent of single-stranded DNA produced by resection of DNA ends. This role is distinct from its roles in recombination. Promotes colocalization of RAD51 and DMC1 during meiotic recombination. Involved in crossover interference. 
Sequence Annotation
 DOMAIN 333 521 Helicase ATP-binding.
 DOMAIN 665 824 Helicase C-terminal.
 NP_BIND 380 387 ATP (Potential).
 MOTIF 506 509 DEGH box.  
Keyword
 ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Isomerase; Meiosis; Nucleotide-binding; Nucleus; Reference proteome; Topoisomerase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 958 AA 
Protein Sequence
MAVISVKPRR REKILQEVKN SSVYQTVFDS GTTQMQIPKY ENKPFKPPRR VGSNKYTQLK 60
PTATAVTTAP ISKAKVTVNL KRSISAGPTL NLAKKPNNLS SNENTRYFTI MYRKPTTKKH 120
KTWSGDGYAT LKASSDKLCF YNEAGKFLGS SMLPSDSDSL FETLFKAGSN EVQLDYELKE 180
NAEIRSAKEA LSQNMGNPSP PTTSTTETVP STKNDGGKYQ MPLSQLFSLN TVKRFKSVTK 240
QTNEHMTTVP KTSQNSKAKK YYPVFDVNKI DNPIVMNKNA AAEVDVIVDP LLGKFLRPHQ 300
REGVKFMYDC LMGLARPTIE NPDIDCTTKS LVLENDSDIS GCLLADDMGL GKTLMSITLI 360
WTLIRQTPFA SKVSCSQSGI PLTGLCKKIL VVCPVTLIGN WKREFGKWLN LSRIGVLTLS 420
SRNSPDMDKM AVRNFLKVQR TYQVLIIGYE KLLSVSEELE KNKHLIDMLV CDEGHRLKNG 480
ASKILNTLKS LDIRRKLLLT GTPIQNDLNE FFTIIDFINP GILGSFASFK RRFIIPITRA 540
RDTANRYNEE LLEKGEERSK EMIEITKRFI LRRTNAILEK YLPPKTDIIL FCKPYSQQIL 600
AFKDILQGAR LDFGQLTFSS SLGLITLLKK VCNSPGLVGS DPYYKSHIKD TQSQDSYSRS 660
LNSGKLKVLM TLLEGIRKGT KEKVVVVSNY TQTLDIIENL MNMAGMSHCR LDGSIPAKQR 720
DSIVTSFNRN PAIFGFLLSA KSGGVGLNLV GASRLILFDN DWNPSVDLQA MSRIHRDGQK 780
KPCFIYRLVT TGCIDEKILQ RQLMKNSLSQ KFLGDSEMRN KESSNDDLFN KEDLKDLFSV 840
HTDTKSNTHD LICSCDGLGE EIEYPETNQQ QNTVELRKRS TTTWTSALDL QKKMNEAATN 900
DDAKKSQYIR QCLVHYKHID PARQDELFDE VITDSFTELK DSITFAFVKP GEICLREQ 958 
Gene Ontology
 GO:0005634; C:nucleus; IC:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003916; F:DNA topoisomerase activity; IEA:UniProtKB-KW.
 GO:0015616; F:DNA translocase activity; IDA:SGD.
 GO:0003690; F:double-stranded DNA binding; IMP:SGD.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0032508; P:DNA duplex unwinding; IMP:SGD.
 GO:0006281; P:DNA repair; IMP:SGD.
 GO:0030491; P:heteroduplex formation; IDA:SGD.
 GO:0045144; P:meiotic sister chromatid segregation; IMP:SGD.
 GO:0007131; P:reciprocal meiotic recombination; IMP:SGD. 
Interpro
 IPR018838; DUF2439.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF10382; DUF2439
 PF00271; Helicase_C
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS