CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-045037
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Splicing factor U2AF 65 kDa subunit 
Protein Synonyms/Alias
  
Gene Name
 U2AF2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61IFQGQSLKIRRPHDYubiquitination[1]
122LTSFGPLKAFNLVKDubiquitination[2]
245CSKYGLVKSIEIPRPubiquitination[3]
263VEVPGCGKIFVEFTSubiquitination[4]
294ANRVVVTKYCDPDSYacetylation[5, 6]
294ANRVVVTKYCDPDSYubiquitination[4]
Reference
 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 307 AA 
Protein Sequence
MMDFFNAQMR LGGLTQAPGN PVLAVQINQD KNFAFLEFRS VDETTQAMAF DGIIFQGQSL 60
KIRRPHDYQP LPGMSENPSV YVPGVVSTVV PDSAHKLFIG GLPNYLNDDQ VKELLTSFGP 120
LKAFNLVKDS ATGLSKGYAF CEYVDINVTD QAIAGLNGMQ LGDKKLLVQR ASVGAKNATL 180
STINQTPVTL QVPGLMSSQV QMGGHPTEVL CLMNMVLPEE LLDDEEYEEI VEDVRDECSK 240
YGLVKSIEIP RPVDGVEVPG CGKIFVEFTS VFDCQKAMQG LTGRKFANRV VVTKYCDPDS 300
YHRRDFW 307 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006397; P:mRNA processing; IEA:InterPro. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR006529; U2AF_lg. 
Pfam
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS