CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004565
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AP-2 complex subunit alpha-1 
Protein Synonyms/Alias
 100 kDa coated vesicle protein A; Adapter-related protein complex 2 alpha-1 subunit; Adaptor protein complex AP-2 subunit alpha-1; Alpha-adaptin A; Alpha1-adaptin; Clathrin assembly protein complex 2 alpha-A large chain; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit 
Gene Name
 Ap2a1 
Gene Synonyms/Alias
 Adtaa; Clapa1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
35AEIKRINKELANIRSacetylation[1]
35AEIKRINKELANIRSubiquitination[2]
165GDSMDSVKQSAALCLubiquitination[2]
277PPEDAAVKGRLVECLubiquitination[2]
403MCDRSNAKQIVSEMLubiquitination[2]
615RESSILAKLKRKKGPubiquitination[2]
810TQLAVQTKRVAAQVDubiquitination[2]
880QDFFQRWKQLSLPLQubiquitination[2]
891LPLQEAQKIFKANHPubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). 
Sequence Annotation
 MOD_RES 626 626 Phosphoserine (By similarity).
 MOD_RES 650 650 Phosphothreonine.
 MOD_RES 652 652 Phosphoserine.
 MOD_RES 653 653 Phosphothreonine.
 MOD_RES 655 655 Phosphoserine.  
Keyword
 Alternative splicing; Cell membrane; Coated pit; Complete proteome; Endocytosis; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 977 AA 
Protein Sequence
MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL DGYSKKKYVC 60
KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV LVNSNSELIR LINNAIKNDL 120
ASRNPTFMCL ALHCIANVGS REMGEAFAAD IPRILVAGDS MDSVKQSAAL CLLRLYKASP 180
DLVPMGEWTA RVVHLLNDQH MGVVTAAVSL ITCLCKKNPD DFKTCISLAV SRLSRIVSSA 240
STDLQDYTYY FVPAPWLSVK LLRLLQCYPP PEDAAVKGRL VECLETVLNK AQEPPKSKKV 300
QHSNAKNAIL FETISLIIHY DSEPNLLVRA CNQLGQFLQH RETNLRYLAL ESMCTLASSE 360
FSHEAVKTHI DTVINALKTE RDVSVRQRAA DLLYAMCDRS NAKQIVSEML RYLETADYAI 420
REEIVLKVAI LAEKYAVDYS WYVDTILNLI RIAGDYVSEE VWYRVLQIVT NRDDVQGYAA 480
KTVFEALQAP ACHENMVKVG GYILGEFGNL IAGDPRSSPP VQFSLLHSKF HLCSVATRAL 540
LLSTYIKFIN LFPETKATIQ GVLRAGSQLR NADVELQQRA VEYLTLSSVA STDVLATVLE 600
EMPPFPERES SILAKLKRKK GPGAASALDD SRRDTSSNDI NGGVEPTPST VSTPSPSADL 660
LGLRAAPPPA APPAPVGGNL LVDVFSDGPT AQPSLGPTPE EAFLSELEPP APESPMALLA 720
DPAPAADPGP EDIGPPIPEA DELLNKFVCK NSGVLFENQL LQIGVKSEFR QNLGRMYLFY 780
GNKTSVQFQN FLPTVVHPGD LQTQLAVQTK RVAAQVDGGA QVQQVLNIEC LRDFLTPPLL 840
SVRFRYGGTA QSLTLKLPVT INKFFQPTEM AAQDFFQRWK QLSLPLQEAQ KIFKANHPMD 900
AEVTKAKLLG FGSALLDNVD PNPENFVGAG IIQTKALQVG CLLRLEPNAQ AQMYRLTLRT 960
SKEPVSRHLC ELLAQQF 977 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0016323; C:basolateral plasma membrane; IEA:Compara.
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
 GO:0030117; C:membrane coat; IDA:MGI.
 GO:0030141; C:secretory granule; TAS:MGI.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0006886; P:intracellular protein transport; TAS:MGI.
 GO:0016192; P:vesicle-mediated transport; TAS:MGI. 
Interpro
 IPR017104; AP2_complex_asu.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR013038; Clathrin_a-adaptin_app_Ig-like.
 IPR003164; Clathrin_a-adaptin_app_sub_C.
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR015873; Clathrin_a/coatomer_app_sub_C.
 IPR009028; Coatomer/calthrin_app_sub_C.
 IPR013041; Coatomer/clathrin_app_Ig-like. 
Pfam
 PF01602; Adaptin_N
 PF02296; Alpha_adaptin_C
 PF02883; Alpha_adaptinC2 
SMART
 SM00809; Alpha_adaptinC2 
PROSITE
  
PRINTS