CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023881
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein phosphatase methylesterase 1 
Protein Synonyms/Alias
 PME-1 
Gene Name
 PPME1 
Gene Synonyms/Alias
 PME1; PP2593; PRO0750 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MSALEKSMHLGRLacetylation[1]
6**MSALEKSMHLGRLubiquitination[2, 3]
217NAIEWSVKSGQIRNLubiquitination[4]
281EDDMETKKDHPYTWRubiquitination[3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme. 
Sequence Annotation
 ACT_SITE 156 156
 ACT_SITE 181 181
 ACT_SITE 349 349
 MOD_RES 27 27 Phosphoserine (By similarity).
 MOD_RES 42 42 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Hydrolase; Phosphoprotein; Reference proteome; Serine esterase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 386 AA 
Protein Sequence
MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF ESMEDVEVEN 60
ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII SRVQCRIVAL DLRSHGETKV 120
KNPEDLSAET MAKDVGNVVE AMYGDLPPPI MLIGHSMGGA IAVHTASSNL VPSLLGLCMI 180
DVVEGTAMDA LNSMQNFLRG RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG 240
ITSPEGSKSI VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG 300
WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA VHEDAPDKVA 360
EAVATFLIRH RFAEPIGGFQ CVFPGC 386 
Gene Ontology
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0051722; F:protein C-terminal methylesterase activity; IDA:HGNC.
 GO:0051721; F:protein phosphatase 2A binding; IDA:HGNC.
 GO:0004864; F:protein phosphatase inhibitor activity; TAS:ProtInc.
 GO:0008601; F:protein phosphatase type 2A regulator activity; TAS:HGNC.
 GO:0006482; P:protein demethylation; IDA:HGNC. 
Interpro
 IPR016812; PPase_methylesterase_euk. 
Pfam
  
SMART
  
PROSITE
 PS00120; LIPASE_SER 
PRINTS